Whole genome sequencing and phylogenetic analysis of feline anelloviruses |
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| Affiliation: | 1. Guangxi Key Lab of Agricultural Resources Chemistry and Biotechnology, College of Chemistry and Food Science, Yulin Normal University, 1303 Jiaoyudong Road, Yulin 537000, PR China;2. Department of Chemistry, Guilin Normal College, 21 Xinyi Road, Gulin 541001, PR China;3. State Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmacy, Guangxi Normal University, 15 Yucai Road, Guilin 541004, PR China;1. Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China;2. State Key Laboratory of Toxicology and Medical Countermeasures, Beijing 100850, China |
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| Abstract: | Torque teno felis virus (FcTTV) was detected in the cat population in the Czech Republic. A total of 110 serum samples were tested by a nested PCR technique using specific primers, situated in the highly conserved untranslated region of the virus genome. The frequency of feline TT virus in the Czech Republic was found to be 33.63%. Sequencing of PCR product from several virus strains showed that all of them are closely related and belong to the same virus species. Whole genome sequencing of three strains was performed to compare overall genetic heterogeneity of feline TT viruses. One of these three strains showed more that 10% difference at the nucleotide level. Furthermore we didn’t find any correlation between FcTTV infection and sex or health status of examined animals. |
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| Keywords: | Anelloviruses Cats Genome sequencing Prevalence |
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