Antimicrobial susceptibility,virulence gene profiles and molecular subtypes of Salmonella Newport isolated from humans and other sources |
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| Institution: | 1. Department of Food Science and Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China;2. Shanghai Municipal Center for Disease Control and Prevention, Shanghai, China;3. Department of Food Science and Nutrition, University of Maryland, College Park, MD, USA;4. Shanghai Municipal Ke-Ma-Jia Technology Center for Microbiology, Shanghai, China;5. Key Laboratory of Veterinary Vaccine Innovation of the Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China;1. Department of Food Science & Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China;2. National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China;3. Shanghai Municipal Center for Disease Control and Prevention, Shanghai, China;4. Shanghai Municipal Ke-Ma-Jia Technology Center for Microbiology, Shanghai, China;5. State Key Lab of Chirosciences, Department of Applied Biology and Chemical Technology, Hong Kong Polytechnic University, Hong Kong, China;6. Joint Institute for Food Safety & Applied Nutrition, Center for Food Safety & Security Systems, Department of Nutrition & Food Science, University of Maryland, MD, USA;1. Universidade Federal de Viçosa, Departamento de Veterinária, Avenida PH Rolfs, s/n, Campus Universitário, 36570-900 Viçosa, MG, Brazil;2. Universidade Federal do Paraná - Setor Palotina, Departamento de Ciências Veterinárias, Rua Pioneiro, 2153, Jardim Dallas, 85950-000 Palotina, PR, Brazil;3. Washington State University, Paul G. Allen School for Global Animal Health, 240 SE Ott Road, PO Box 647090, 99164-7040 Pullman, WA, United States |
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| Abstract: | Salmonella Newport (S. Newport) is a major serotype associated with human salmonellosis. A total of 79 S. Newport recovered from humans and other sources in China were characterized for antimicrobial susceptibility, virulence gene profiles and molecular subtypes using pulsed field gel electrophoresis (PFGE). Approximately 63.3% of the isolates were susceptible to all of 16 antimicrobials tested. Nearly one third of the isolates (31.6%) were resistant to sulfisoxazole, 20.3% to tetracycline and 13.9% to nalidixic acid. Twelve isolates (15.2%) were resistant to three or more antimicrobials. Among 10 virulence genes detected, Salmonella pathogenicity island genes avrA, ssaQ, mgtC, siiD, and sopB and fimbrial gene bcfC were present in most of the isolates (93.7% to 100%). Overall, we observed nine distinct virulence gene profiles, three of which (VP1, VP2 and VP3) were most common (86.1%). A total of 56 PFGE patterns were identified and mainly grouped into seven clusters (A to G) with 80% pattern similarity. Isolates from aquatic product shared a high similarity with those from humans in several clusters, highlighting a potential risk of aquatic product as a source of S. Newport that infect humans. Furthermore, there was a strong association between certain PFGE clusters and virulence gene profiles, suggesting virulence subtyping can be a useful epidemiological tool to discriminate S. Newport isolates. |
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