Genetic analysis of Giardia and Cryptosporidium from people in Northern Australia using PCR-based tools |
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| Institution: | 1. Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Victoria, Australia;2. Pathology Queensland, Townsville Hospital, Townsville, Queensland, Australia;3. School of Medical and Applied Sciences, Central Queensland University, Rockhampton, North Rockhampton, Queensland, Australia;4. Melbourne Water Corporation, Victoria, Australia;5. Institute of Parasitology, Department of Pathobiology, University of Veterinary Medicine Vienna, Austria;1. College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China;2. State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Chinese Academy of Agricultural Sciences, Lanzhou Veterinary Research Institute, Lanzhou 730046, China;3. Key Laboratory of Veterinary Public Health of Yunnan Province, College of Veterinary Medicine, Yunnan Agricultural University, Kunming 650201, China;4. Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University College of Veterinary Medicine, Yangzhou 225009, China;1. Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria 3010, Australia;2. Melbourne Water, Docklands, Victoria 3008, Australia;3. The Walter and Eliza Hall Institute, Parkville, Victoria 3052, Australia |
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| Abstract: | To date, there has been limited genetic study of the gastrointestinal pathogens Giardia and Cryptosporidium in northern parts of Australia. Here, PCR-based methods were used for the genetic characterization of Giardia and Cryptosporidium from 695 people with histories of gastrointestinal disorders from the tropical North of Australia. Genomic DNAs from fecal samples were subjected to PCR-based analyses of regions from the triose phosphate isomerase (tpi), small subunit (SSU) of the nuclear ribosomal RNA and/or the glycoprotein (gp60) genes. Giardia and Cryptosporidium were detected in 13 and four of the 695 samples, respectively. Giardia duodenalis assemblages A and B were found in 4 (31%) and 9 (69%) of the 13 samples in persons of < 9 years of age. Cryptosporidium hominis (subgenotype IdA18), Cryptosporidium mink genotype (subgenotype IIA16R1) and C. felis were also identified in single patients of 11–21 years of age. Future studies might focus on a comparative study of these and other protists in rural communities in Northern Australia. |
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