| γ-H2AX与DNA双链断裂关系的研究进展 |
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| 引用本文： | 宾萍,郑玉新.γ-H2AX与DNA双链断裂关系的研究进展[J].卫生研究,2007,36(4):520-522. |
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| 作者姓名： | 宾萍 郑玉新 |
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| 作者单位： | 中国疾病预防控制中心职业卫生与中毒控制所,北京,100050 |
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| 基金项目： | 国家重点基础研究发展计划(973计划);国家自然科学基金 |
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| 摘 要： | H2AX是组蛋白的一个种类,普遍存在于整个基因组中。据报道,DNA双链断裂后可诱导位于丝氨酸-139位C-端保守区域内的H2AX磷酸化形成γ-H2AX,在荧光显微镜下形成可见的γ-H2AX焦点。用特异性抗体的免疫荧光法检测γ-H2AX已成为测定DSB的金标准。目前,多种物理性、化学性和生物性的因素都能诱导形成γ-H2AX焦点。本文综述了不同因素诱导形成γ-H2AX的机制及其与DNA双链断裂之间的关系的研究进展。
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| 关 键 词： | DNA损伤 γ-H2AX DNA双链断裂 |
| 文章编号： | 1000-8020（2007）04-0520-03 |
| 修稿时间： | 2006-08-28 |
Association between γ-H2AX and DNA double-strand breaks |
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| BIN Ping,ZHENG Yuxin.Association between γ-H2AX and DNA double-strand breaks[J].Journal of Hygiene Research,2007,36(4):520-522. |
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| Authors: | BIN Ping ZHENG Yuxin |
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| Institution: | National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China |
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| Abstract: | H2AX may be one family of histone and distributes among the genome. It is reported that DNA double-strand breaks (DSB) could induce phosphorylation of H2AX at conservative C-terminal region of serine 139 and the formation of gamma-H2AX. The gamma-H2AX foci can be observed by the fluorescence microscope. An immunofluorescence assay with specific antibodies recognizing gamma-H2AX could become the gold standard for detecting the DSB. It was indicated that some of physical, chemical, and biological factors could induce the formation of the gamma-H2AX foci. In this paper, the advance of the studies of mechanism of gamma-H2AX formation and the association between gamma-H2AX and DSB were reviewed. |
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| Keywords: | DNA damage γ-H2AX DNA double-strand breaks |
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