肿瘤及多种疾病患者血清P53蛋白和抗体检测

目的检测不同疾病患者血清P53蛋白和抗体的阳性率。方法采用间接酶联免疫吸附试验（ELISA）法检测血清P53抗体，夹心ELISA法检测P53蛋白。结果血清P53蛋白总阳性率为3．73％，抗体总阳性率为12．98％。恶性肿瘤、良性肿瘤、非肿瘤性疾病患者的P53蛋白阳性率分别为4．20％、0％及3．74％；P53抗体阳性率分别为14．23％、4．41％及13．02％。卵巢癌阳性率最高（41．67％），其次为鼻咽癌（33．33％）、白血病（24．24％）。肝硬化、慢性乙型肝炎、慢性胆囊炎、结肠息肉、慢性阑尾炎等患者中也发现有血清P53蛋白和抗体阳性。结论血清P53蛋白和抗体作为一种无创性检测指标可用于肿瘤的辅助诊断及早期发现，但其实际应用价值尚需进一步评价。

Detection of serum P53 protein and P53 antibody in different patients

Objective To detect the positive rate of serum P53 protein and P53 antibody in different patients, Methods Indirect ELISA was adopted to detect serum P53 antibody, and double-antibodies sandwich ELISA technique was used to measure serum P53 protein. Results The total positive rate of serum P53 protein and P53 antibody was 3.74 % and 13.02 % respectively. The positive rates of serum P53 protein and P53 antibody in patients with malignant tumor and benign tumor, non-neoplastic disease were 4.20%, 14.23% ; 0%, 4.41% ; and 3.74%, 13.02% respectively. The highest positive rate was found in patients with ovarian cancer （41. 67%）, followed by nasopharyngeal carcinoma （33.33%）, leukemia （24.24 %）. Serum P53 protein and P53 Ab were also present in patients with hepatocirrhosis, chronic hepatitis B, chronic cholecystitis, colonic polyp, chronic appendicitis and so on. Conclusion As suitable noninvasive detecting parameters, serum P53 protein and P53 antibody may be applied in tumor screening and early detection.