铅致幼鼠海马回及附近皮质细胞凋亡的实验研究

目的 从细胞凋亡的的角度探讨铅的发育神经毒性。方法 大鼠孕期及哺乳期不同剂量染铅，用原子吸收分光光度法测定仔鼠血、脑铅含量，取海马回及附近皮质用TUNEL法测定凋亡细胞数量。结果 染铅使出生后7天幼鼠海马回及附近皮质的细胞凋亡数量增加，但对出生后21天幼鼠的影响不明显。结论 铅可通过增加海马回及附近皮质细胞凋亡数量而表现出发育神经毒性。

The experimental study on apoptosis of hippocampus and cortex cells in rat offsprings exposed to lead

Objective To study the developmental neurotoxicity.Method The offsprings of rats exposed to lead during pregnant and breast feeding period were used to determine the lead content in blood and brain,the apoptotic cells in hippocampus and cortex were also assayed by TUNEL method.Result The number of apoptotic cells in hippocampus and nearby cortex increased in the young rats at the 7th day after birth,but no obvious change in those at the 21st day after birth.Conclusion Lead could induce developmental neurotoxicity by excessive apoptosis of hippocampus and nearby cortex cells during developmental period.