内毒素致兔肝线粒体膜磷脂含量改变及阳离子A的拮抗效应

目的： 采用内毒素血症兔实验模型，研究肝线粒体膜磷脂含量的改变及阳离子A的拮抗效应。方法: 将48只日本大耳白兔随机分为正常对照组(Ⅰ组)、内毒素组(Ⅱ组)和阳离子A拮抗组(Ⅲ组)。3组同时进行相应处理后，分别在第3、7 h 测定各组肝线粒体膜中磷脂酰胆碱(PC)、磷脂酰乙醇胺(PE)、磷脂酰丝氨酸(PS)和磷脂酰肌醇(PI)的含量变化。结果: Ⅱ组中PC、PE、PS、PI含量在第 3 h、7 h 均低于Ⅰ组，差异极显著(P<0.01)；而Ⅲ组中4种磷脂的含量在第 3 h、7 h 又均高于Ⅱ组(P<0.05, P<0.01)。结论: 内毒素致使兔肝线粒体膜磷脂含量降低，而阳离子A一定程度上具有对内毒素的拮抗作用和对肝线粒体膜的保护效应。

Effect of endotoxin on phospholipids of hepatic mitochondria membrane and cation A antagonism in rabbits

AIM: To investigate the effect of endotoxin on phospholipid of hepatic mitochondria membrane (MiM) and cation A (CA) antagonism in the model of endotoxemia in rabbits. METHODS: Forty-eight healthy Japanese big-ear rabbits were randomly divided into 3 groups: the normal control (groupⅠ), endotoxin treatment group (groupⅡ) and cation A and endotoxin treatment group (group Ⅲ). The contents of phosphatidylcholine (PC), phosphatidylethanolamin (PE), phosphatidylserine (PS) and phosphatidylinositol (PI) in MiM of three groups were measured at the 3rd or 7th hour after corresponding treatment. RESULTS: The contents of the four phospholipids in groupⅡ were lower than those in groupⅠ during the experiment (P<0.01). A significant increase in the contents of four phospholipids in group Ⅲ was observed as compared to group Ⅱ (P<0.05, P<0.01). CONCLUSION: Endotoxin decreases the major membrane phospholipids in MiM, whereas CA has a prominent protective effect on injuries by endotoxin in MiM and provides a valuable evidence for the use of some drugs of endotoxin antagonist.