沙棘汁对环磷酰胺所致大鼠血小板减少的影响

目的:观察沙棘汁(JFH)对血小板功能、质量的影响。方法:Wistar大鼠48只,随机分成空白对照组、造模组、益血生胶囊(YSJN)组、沙棘汁组。采用环磷酰胺30mg/kg每日腹腔注射(连续3d)复制成血小板减少模型,然后分组进行治疗。持续11d。于造模后第2、4、6和8d进行血小板计数,并同时检测血小板平均体积(MPV)、血小板体积变异系数(PDW),第8d心脏取血检测血小板内3’,5’一环-磷酸腺苷(cAMP)、3’,5’一环-磷酸鸟苷(cGMP)及血小板聚集功能等。结果:沙棘汁可抑制凝血时间的缩短,使血小板数量增高,加强血小板聚集功能,同时降低血小板内cGMP含量。结论:沙棘汁具有促进血小板功能、改善血小板质量、防止血小板下降和促进血小板数量恢复的功效。

Effects of the juice of Fructus Hippophae (JFH) on thrombocytopenia induced by cyclophosphamide in rats

AIM: To investigate the effects of the juice of fructus hippophae (JFH) on thrombocytopenia in rats. METHODS: Forty-eight wistar rats were divided into control group, model group, Yixuesheng-Jiaonang (YSJN) group and JFH group. Rats were injected intraperitoneally with cyclophosphamide (CTX, 30 mg/kg) or saline once a day for 3 days. And then, Saline, YSJN or JFH was administered (ig) once a day for 11 days. On the second, fouth, sixth and eighth day, the thrombocyte were counted, the mean platelet volume (MPV) and PDW were examined. On the eighth day, the cAMP, cGMP in platelets and platelet aggregation function were also examined. RESULTS: JFH could shorten the time of blood coagulation, increase the count of platelet, and enhance the platelet aggregation function. The experiment also showed that the JFH could decrease cGMP content in platelet. CONCLUSION: JFH could enhance blood coagulation and quality of platelet and prevent thrombocytopenia induced by cyclophosphamide in rats.