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| 抗幽门螺旋杆菌尿素酶B亚单位(UreB)单克隆抗体抗原识别表位的初步鉴定 | |
| 引用本文： | 李海侠,吴亚男,王宪灵,张卫军,罗平,余抒,陈洪章,毛旭虎. 抗幽门螺旋杆菌尿素酶B亚单位(UreB)单克隆抗体抗原识别表位的初步鉴定[J]. 免疫学杂志, 2007, 23(2): 184-186,190 |
| 作者姓名： | 李海侠 吴亚男 王宪灵 张卫军 罗平 余抒 陈洪章 毛旭虎 |
| 作者单位： | 第三军医大学医学检验系临床微生物及免疫学教研室暨重庆市生物制药工程技术研究中心,重庆,400038 |
| 摘 要： | 目的 初步确定抗幽门螺杆菌尿素酶B亚单位(UreB)单克隆抗体(mAb)6E6识别的抗原表位.方法 采用截短法分段构建含UreB抗原的重组质粒,分别命名为U12,U13,U15,U16,U47.用IPTG诱导表达融合蛋白.对表达产物进行SDS-PAGE蛋白电泳及Western blot 分析.结果 6E6能够分别识别1～300位氨基酸的U12片段,1～260位氨基酸的U16片段,1～230位氨基酸的U15片段,而不能识别1～200位氨基酸的U13片段和251～389位氨基酸的U47片段.结论 mAb 6E6抗体识别的抗原表位位于200～230位氨基酸. |
| 关 键 词： | 幽门螺杆菌 UreB分段抗原分子 单克隆抗体 抗原表位 抗幽门螺旋杆菌 幽门螺杆菌尿素酶 亚单位 UreB 单克隆抗体 抗原表位 识别表位 Helicobacter pylori urease B subunit epitope characterization 抗体识别 氨基酸 结果 分析 Western blot 蛋白电泳 诱导表达 融合蛋白 |
| 文章编号： | 1000-8861（2007）02-0184-04 |
| 修稿时间： | 2006-09-182006-12-15 |
Elementary characterization on epitope of urease B subunit of Helicobacter pylori |
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| LI Hai-xia,WU Ya-nan,WANG Xian-ling,ZHANG Wei-jun,LUO Ping,YU Shu,CHEN Hong-zhang,MAO Xuhu. Elementary characterization on epitope of urease B subunit of Helicobacter pylori[J]. Immunological Journal, 2007, 23(2): 184-186,190 | |
| Authors: | LI Hai-xia WU Ya-nan WANG Xian-ling ZHANG Wei-jun LUO Ping YU Shu CHEN Hong-zhang MAO Xuhu |
| Affiliation: | Department of Clinical Microbiology and Immunology, College of Medical Laboratory, Third Military Medical University, Chongqing 400038, China |
| Abstract: | Objective To definite the epitope domain of monoclonal antibody 6E6 of ureB Helicobacter pylori. Methods Recombinant plasmids U12, U13, U15, U16, and U47 were constructed, and then transformed into E.coli. BL21 (DE3) for expression under IPTG induction. The expression proteins were identified by SDS-PAGE and Western blotting. Results The monoclonal antibody 6E6 could recognize antigens of U12 (amino acids from 1 to 300), U15(amino acids from 1 to 230), and U16 (amino acids from 1 to 260), but could not recognize the antigen of U13 (amino acids from 1 to 200) or U47 (amino acids from 251 to 389). Conclusion The epitope of monoclonal antibody 6E6 is located in 200-230aa. |
| Keywords: | Helicobacter Fylori UreB fragment antigen Monoclonal antibody Epitope |
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