自体造血干细胞移植对系统性红斑狼疮患者免疫功能的影响

为探讨自体造血干细胞移植对系统性红斑狼疮(SLE)患者免疫学指标的影响并评估其治疗效果，应用流式细胞术、酶联免疫吸附试验等方法动态监测33例SLE患者移植前及移植后1，3，6，12个月时白细胞分化抗原，sIL-2R，IL-6，C3，C4，自身抗体，免疫球蛋白等免疫学指标变化。结果显示：移植后CD4^ ，CD19^ 细胞持续减低，CD8^ ，CD16^ CD56^ 细胞持续回升；sIL-2R，IL-6明显下降，C3，C4显著回升，自身抗体阳性率显著下降；移植后26例完全缓解，3例部分缓解，4例移植后46个月复发。结论：SLE患者自体造血干细胞移植后免疫学指标发生显著改变，这些结果可作为判断SLE不同缓解状态的免疫学预测指标。

Effects of Autologous Hematopoietic Stem Cell Transplantation on Immune Function in Patient with Systemic Lupus Erythematosus

To investigate the effects of autologous hematopoietic stem cell transplantation (AHSCT) on immune index in patient with systemic lupus erythematosus (SLE), and evaluate its treatment outcome, the flow cytometry (FCM) and enzyme linked immunsorbent assay (ELISA) were used to detect the leukocyte differentiation antigen, sIL-2R, IL-6, C3, C4, autoantibodies, immunoglobulin for 33 case of SLE before transplantation and at 1,3,6,12 months after transplantation. The results showed that the ratio of CD4 ,CD19 cell, the level of sIL-2R, IL-6 and the positive rate of autoantibodies were significantly lower, CD8 ,CD16 CD56 cell and C3,C4 were higher than those before transplantation. Out of the 33 patients, 26 achieved CR, 3 reached PR and 4 relapsed at 4-6 months after transplantation. It is concluded that the immune indexes of patients with SLE changed significantly following AHSCT. These immune indexes may be indications to predict the status of remission in patient with SLE.