| 寡核苷酸探针RNA原位杂交法检测肺孢子虫的研究 |
| |
| 引用本文： | 常志尚,张忠广,宫玉香. 寡核苷酸探针RNA原位杂交法检测肺孢子虫的研究[J]. 热带医学杂志, 2011, 11(10): 1115-1117 |
| |
| 作者姓名： | 常志尚 张忠广 宫玉香 |
| |
| 作者单位： | 1. 青岛大学医学院病原生物学实验室,山东青岛,266071
2. 青岛大学医学院寄生虫学教研室,山东青岛,266071 |
| |
| 摘 要： | 目的探讨RNA原位杂交法在肺孢子虫病理检测中的应用。方法采用小亚单位RNA位点来源的寡核苷酸探针、地高辛加尾标记、Wistar雌性大鼠皮下注射地塞米松建立肺孢子虫肺炎动物模型,取肺组织制备石蜡标本进行RNA原位杂交,并将结果与瑞氏-吉姆萨染色法进行比较。结果 RNA原位杂交法于感染后第3周检到虫体,呈游离分布;7～8周时检测到大量包囊;9～10周时滋养体大量出现;组织内肺孢子虫的检出率(32/32)高于瑞氏-吉姆萨染色法(25/32)。结论 RNA原位杂交法是一种敏感特异的肺孢子虫病理检测方法。
|
| 关 键 词： | 肺孢子虫 诊断 RNA原位杂交 |
Detection of Pneumocystis carinii on the lung paraffin section by RNA in situ hybridization |
| |
| CHANG Zhi-shang,ZHANG Zhong-guang,GONG Yu-xiang. Detection of Pneumocystis carinii on the lung paraffin section by RNA in situ hybridization[J]. Journal Of Tropical Medicine, 2011, 11(10): 1115-1117 |
| |
| Authors: | CHANG Zhi-shang ZHANG Zhong-guang GONG Yu-xiang |
| |
| Affiliation: | CHANG Zhi-shang,ZHANG Zhong-guang,GONG Yu-xiang (Department of Human Micromorphology,Medical College of Qingdao University,Shandong,Qingdao 266071,China) |
| |
| Abstract: | Objective To investigate the possible application of RNA in situ hybridization for the detection of Pneumocystis carinii. Methods The Pneumocystis carinii infected Wistar rat model was established. Rats inoculated with Pneumocystis carinii were sacrificed 3, 4, 5, 6, 7, 8, 9 and 10 weeks after injection with Dexanethasone, and the paraffin sections of lung were prepared to perform the in situ hybridization with Dig-labeled oligonucleotide probe complememtary to small subunit rRNA of Pneumocystis carinii. Re... |
| |
| Keywords: | Pneumocystis carinii diagnose RNA in situ hybridization |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
