| 基因修饰树突状细胞诱导前列腺癌患者外周血T细胞亚群重排的研究 |
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| 引用本文： | 宋浩杰,涂小玉,严晓,尤长宣,罗荣城,吕成伟.基因修饰树突状细胞诱导前列腺癌患者外周血T细胞亚群重排的研究[J].热带医学杂志,2011,11(11):1223-1225,1266. |
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| 作者姓名： | 宋浩杰 涂小玉 严晓 尤长宣 罗荣城 吕成伟 |
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| 作者单位： | 南方医科大学南方医院肿瘤中心,广东广州,510515 |
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| 基金项目： | 国家自然科学基金(81071847); 广东省科技计划项目(2008B030301156); 南方医院院长基金(2008B012) |
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| 摘 要： | 目的探讨以腺相关病毒(AAV)为载体,前列腺特异性抗原(PSA)基因转染树突状细胞(DC)诱导前列腺癌患者外周血T细胞亚群变化特点及临床意义。方法抽取30例前列腺癌患者外周血,采用密度梯度离心法分离外周血单个核细胞,以rAAV/PSA感染DC前体细胞,采用系列细胞因子诱导DC前体细胞成熟。第6天收集成熟DC并与T细胞按比例混合培养,诱导细胞毒性T淋巴细胞(CTL)。分别于DC与T细胞混合培养前后应用流式细胞术分析外周血T细胞亚群及调节性T细胞(CD4+CD25+FoxP3+Treg)的表达水平。结果 PSA基因转染DC刺激T淋巴细胞爆发增殖,与培养前比较,混合培养6d后CD8+、CD8+CD69+、CD8+CD28+T细胞的比例和CD8+/CD4+比值均明显增高,差异有统计学意义(P<0.01);而CD8+CD28-T细胞和Treg细胞的比例均显著降低,差异有统计学意义(P<0.01)。CD4+T细胞比例较前略有升高,但差异无统计学意义(P>0.05)。结论 PSA基因转染DC能够有效地激活CD8+抗原特异性CTL,下调免疫抑制性T细胞,提高患者的细胞免疫功能,为前列腺癌的免疫治疗提供新的有效策略。
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| 关 键 词： | 树突状细胞 基因转染 前列腺癌 T淋巴细胞亚群 调节性T细胞 |
Gene-transducted dendritic cells induce T lymphocyte subsets repopulation in peripheral blood of patients with prostate cancer |
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| SONG Hao-jie,TU Xiao-yu,YAN Xiao,YOU Chang-xuan,LUO Rong-cheng,Lü Cheng-wei.Gene-transducted dendritic cells induce T lymphocyte subsets repopulation in peripheral blood of patients with prostate cancer[J].Journal Of Tropical Medicine,2011,11(11):1223-1225,1266. |
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| Authors: | SONG Hao-jie TU Xiao-yu YAN Xiao YOU Chang-xuan LUO Rong-cheng Lü Cheng-wei |
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| Institution: | SONG Hao-jie,TU Xiao-yu,YAN Xiao,YOU Chang-xuan,LUO Rong-cheng,LU Cheng-wei(Department of Oncology,Nanfang Hospital,Southern Medical University,Guangdong,Guangzhou 510515,China) |
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| Abstract: | Objective To investigate the characteristics of rAAV/PSA-transducted dendritic cells(DCs) to induce T lymphocyte subsets repopulation in peripheral blood of patients with prostate cancer,and its clinical significance.Methods 30 patients with prostate cancer were included in this study.Peripheral blood mononuclear cells(PBMC,the DC precursor cells and lymphocytes) were isolated from prostate cancer patients by density gradient centrifugation and infected with rAAV/PSA virus.Maturation of the DC precursor was... |
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| Keywords: | dendritic cells gene transduction prostate cancer T lymphocyte subset regulatory T cells(Treg) |
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