| 乙型肝炎病毒P区耐药突变与基因型及临床相关性分析 |
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| 引用本文： | 向华国,杨晓丽,刘小君.乙型肝炎病毒P区耐药突变与基因型及临床相关性分析[J].中国热带医学,2011,11(12):1436-1437. |
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| 作者姓名： | 向华国 杨晓丽 刘小君 |
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| 作者单位： | 深圳市宝安区福永人民医院,广东深圳,518103 |
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| 基金项目： | 深圳市科技计划项目(No.201103042) |
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| 摘 要： | 目的探讨HBV的P基因耐药突变的分布特征以及与基因型、临床的相关性。方法对慢性乙肝患者的血液样本进行P基因核苷类似物作用靶区进行聚合酶链反应,扩增产物进行测序,比较序列差异性,同时对其进行基因分型检测。结果 79例乙型肝炎患者共检测出B基因型61例（79.2%）,C基因型18例（20.8%）。通过对HBV-DNA进行P区测序表明耐药突变以拉米夫定耐药为主,为27例（51.9%）;其中B基因型对拉米夫定和阿德福韦的耐药率分别为31.1%（19/61）和9.8%（7/61）,二者交叉耐药率为6.6%（4/61）;而C基因型对拉米夫定和阿德福韦的耐药率分别为44.4%（8/18）和22.2%（4/18）,二者交叉耐药率为16.7%（3/18）,明显高于B型基因（P〈0.05）。慢性肝炎患者中,B型显著多于C型,为89.4%（59/66）;肝癌、肝硬化等重症中,B型感染者则明显少于C型感染者,为15.4%（2/13）（P〈0.05）。结论 79例患者HBV基因型以B型为主,C型次之,耐药突变主要以拉米夫定耐药为主,临床与基因型及耐药突变有显著性关联。
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| 关 键 词： | 乙型肝炎病毒 基因型 聚合酶链反应 |
Correlation of mutations of HBV precore region in hepatitis B patient with HBV genotype and drug resistance |
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| XIANG Hua-guo
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YANG Xiao-li
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LIU Xiao-jun.Correlation of mutations of HBV precore region in hepatitis B patient with HBV genotype and drug resistance[J].China Tropical Medicine,2011,11(12):1436-1437. |
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| Authors: | XIANG Hua-guo YANG Xiao-li LIU Xiao-jun |
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| Institution: | XIANG Hua-guo,YANG Xiao-li,LIU Xiao-jun.(Baoan District Fuyong People,s Hospital,Shenzhen 518103,Guangdong,P.R.China) |
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| Abstract: | Objective To study the correaltion of mutations in the HBV precore region of hepatitis B virus(HBV)DNA with HBV genotype and drug resisntace.Methods The HBV HBV precore region in serum samples from hepatitis B patients were detected by polymerase chain reaction PCR)and HBV gene sequencing and typing were performed after amplification.Results HBV genotype in 79 hepatitis B patients were detected,61(79.2%)were genotype B,18(20.8%)were genotypes C.The resisntace to lamivudine was 51.9%(27/71)highest among the ... |
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| Keywords: | Hepatitis B virus Genotype Polymerase chain reaction |
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