大鼠微量全血彗星实验方法探讨

目的采用全血代替分离出的淋巴细胞作为实验对象,对于彗星实验方法中遇到的问题进行讨论。方法选择大鼠作为实验动物,采用腹腔注射体内染毒24h,尾部采血进行彗星实验。生理盐水染毒组为阴性对照组,环磷酰胺染毒组为阳性对照组（45μg/kg）。结果全血彗星实验结果和分离淋巴细胞实验结果无显著差别（P〉0.05）;随着电泳条件中电压的升高,电泳时间的延长及裂解时间的延长,彗星尾长增长。用乙醇脱水法可保存彗星实验片子,与当天阅片结果无显著性差异。结论微量全血彗星实验方法更简便,可用于大规模的筛选和检测工作。

Trace quantities of whole blood on the experimental method of the comet assay

Objective The problem of experimental method was discussed with whole blood and lymphocytes of rat by comet assay.Methods Tail blood was collected to test by comet assay after 24h intraperitoneal injection.Normal saline treated group used as the negative control.Cyclophosphamide treated group used as the positive control as treated（45μg/kg）.Results There was no marked difference between the result of blood comet and lymphocytes comet（P0.05）.With the rising of electrophoresis voltage and pyrolysis time,comet tail length increased.And there is no marked difference in result read on the same day and saved by ethanol dehydration.Conclusion The results indicated that comet assay with trace quantities of whole blood can be used in screening.