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| 一期凝固法测定尿液组织因子促凝活性的方法学建立 | |
| 引用本文： | 付文金,郑磊,邓任堂,陈楚甜,叶长钦,王少波,刘亮,陈梅莲,彭兰芬,熊石龙,包杰,汤惠华,王前. 一期凝固法测定尿液组织因子促凝活性的方法学建立[J]. 热带医学杂志, 2011, 11(10): 1134-1137 |
| 作者姓名： | 付文金 郑磊 邓任堂 陈楚甜 叶长钦 王少波 刘亮 陈梅莲 彭兰芬 熊石龙 包杰 汤惠华 王前 |
| 作者单位： | 1. 南方医科大学南方医院,广东广州510282;东莞市厚街医院,广东东莞523945 2. 南方医科大学南方医院,广东广州,510282 3. 东莞市厚街医院,广东东莞,523945 |
| 基金项目： | 东莞市重点课题项目(200910515000104) |
| 摘 要： | 目的 建立一种简便检测尿液组织因子促凝血活性的方法.方法 以owren-koller稀释液5倍稀释尿液后,在STAGO全自动凝血仪检测凝固时间,通过兔脑粉不同浓度及其对应的凝固时间确定相应的标准曲线,计算出尿液组织因子(TF)的促凝血活性(TF-PCA),并对该方法进行评价.结果 通过owren-koller稀释液处理... |
| 关 键 词： | 一期凝同法 尿液 组织因子促凝血活性 |
Analysis of urinary tissue factor procoagulant activity |
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| FU Wen-jin,ZHENG Lei,DENG Ren-tang,CHEN Chu-tian,YE Chang-qin,WANG Shao-bo,LIU Liang,CHEN Mei-lian,PENG Lan-fen,XIONG Shi-long,BAO Jie,TANG Hui-hua,WANG Qian. Analysis of urinary tissue factor procoagulant activity[J]. Journal Of Tropical Medicine, 2011, 11(10): 1134-1137 | |
| Authors: | FU Wen-jin ZHENG Lei DENG Ren-tang CHEN Chu-tian YE Chang-qin WANG Shao-bo LIU Liang CHEN Mei-lian PENG Lan-fen XIONG Shi-long BAO Jie TANG Hui-hua WANG Qian |
| Affiliation: | FU Wen-jin1,2,ZHENG Lei1,DENG Ren-tang2,CHEN Chu-tian2,YE Chang-qin2,WANG Shao-bo2,LIU Liang2,CHEN Mei-lian2,PENG Lan-fen2,XIONG Shi-long1,BAO Jie1,TANG Hui-hua2,WANG Qian1 (1.Nanfang Hospital,Southern Medical University,Guangdong,Guangzhou 510282,2. Houjie Hospital of Dongguan City,Dongguan 523945,China) |
| Abstract: | Objective To establish a simple method to analyze the urinary tissue factor procoagulant activity(TF-PCA). Methods Urine was diluted with owren-koller and then urinary TF-PCA was analyzed by one-stage coagulantation with STAGO auto-analyzer.The standard curve between PCA and coagulation time was determined by the different concentration of TF.The method evaluation was conducted. Results Neutralizing monoclonal antibody of TF could effectively inhibit the PCA of urine, so it could verify the PCA originated f... |
| Keywords: | one-stage coagulantation urine TF-PCA |
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