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| HBV转基因小鼠CD4+CD25+调节性T细胞免疫抑制功能的研究 | |
| 引用本文： | 赵光艳,胡显芳,郑紫丹,李彦. HBV转基因小鼠CD4+CD25+调节性T细胞免疫抑制功能的研究[J]. 热带医学杂志, 2011, 11(12): 1371-1374 |
| 作者姓名： | 赵光艳 胡显芳 郑紫丹 李彦 |
| 作者单位： | 1. 重庆市涪陵区人民医院内一科,重庆,408000 2. 重庆市急救医疗中心消化科,重庆,400014 3. 第三军医大学西南医院消化科,重庆,400038 |
| 摘 要： | 目的 通过对HBV转基因小鼠CD4+、CD4+CD25+调节性T细胞数量和免疫抑制功能的研究,探讨CD4+CD25+调节性T细胞在乙肝免疫耐受中的作用.方法 用流式细胞术对12只HBV转基因小鼠和12只正常小鼠外周血CD4+、CD4+CD25+调节性T细胞的频率进行检测;磁珠分选小鼠脾CD4+CD25-T细胞和CD4+CD25+T细胞,分为HBsAg刺激组和ConA刺激组体外单独和共培养,ELISA方法检测诱生的细胞因子IL-2.结果 与正常小鼠比较,HBV转基因小鼠外周血CD4+、CD4+CD25+调节性T细胞数量差异无统计学意义(P＞0.05).HBsAg刺激组,CD4+CD25-T细胞单独或共培养,HBV转基因小鼠CD4+CD25-T细胞诱生IL-2水平显著低于正常小鼠(P＜0.01)；ConA刺激组,HBV转基因小鼠CD4+CD25-T细胞诱生IL-2水平与正常小鼠相比则差异无统计学意义(P＞0.05)；两组小鼠CD4+CD25-T细胞单独培养时诱生IL-2水平均显著高于共培养(P＜0.01).结论 与正常小鼠比较,HBV转基因小鼠CD4+、CD4+CD25+调节性T细胞数量和免疫抑制功能差异无统计学意义,但T细胞水平对乙肝病毒存在特异性免疫耐受.CD4+CD25+调节性T细胞可抑制CD4+、CD8+T细胞. |
| 关 键 词： | HBV转基因小鼠 CD4+CD25+调节性T细胞 慢性乙型肝炎 免疫耐受 |
Study on the functions of CD4~+CD25~+ regulatory T cells in HBV transgenic mice |
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| ZHAO Guang-yan , HU Xian-fang , ZHENG Zi-dan , LI yan. Study on the functions of CD4~+CD25~+ regulatory T cells in HBV transgenic mice[J]. Journal Of Tropical Medicine, 2011, 11(12): 1371-1374 | |
| Authors: | ZHAO Guang-yan HU Xian-fang ZHENG Zi-dan LI yan |
| Affiliation: | ZHAO Guang-yan1,HU Xian-fang1,ZHENG Zi-dan2,LI yan3 (1.First Department of Internal Medicine,Fuling District People's Hospital of Chongqing,Chongqing 408000,2.Department of Gastroenterology,Chongqing Emergency Medical Center,Chongqing 400014,3. Department of Gastroenterology,Southwest Hospital,Third Military Medical University,Chongqing 400038,China) |
| Abstract: | Objective To study the role of CD4+ and CD4+CD25+ regulatory T cells in HBV transgenic mice in the host immune tolerance to HBV. Methods Flow cytometry was used to analyze the frequencies of peripheral blood CD4+T cells and CD4+CD25+ regulatory T cells from 12 HBV transgenic mice and 12 normal mice. CD4+CD25+ regulatory T cells and CD4+CD25- T cells were isolated from HBV transgenic mice splenocytes using a CD4+CD25+ regulatory T cells isolation kit,and then cultured alone or co-cultured in vitro in the HBs... |
| Keywords: | HBV transgenic mice CD4 CD25 regulatory T cell chronic hepatitis B immune tolerance |
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