| 2009年海南省甲型H1N1流感病毒核酸检测结果 |
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| 引用本文： | 曾祥洁,潘家兴,马焱,李臻,李丹丹,林春燕,邱丽.2009年海南省甲型H1N1流感病毒核酸检测结果[J].中国热带医学,2011,11(10):1253-1254. |
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| 作者姓名： | 曾祥洁 潘家兴 马焱 李臻 李丹丹 林春燕 邱丽 |
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| 作者单位： | 海南省疾病预防控制中心,海南 海口,570203 |
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| 基金项目： | 海南省自然科学基金立项课题(No.806100) |
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| 摘 要： | 目的及时了解海南省2009年流感样病例及其密切接触者中甲型H1N1流感病毒感染情况。方法应用实时荧光定量PCR法对呼吸道鼻咽拭子标本中的流感病毒核酸进行定性检测。结果全年共检测哨点监测和爆发疫情标本1 148份,荧光定量PCR法检测结果：流感病毒A型核酸阳性为686份,B型核酸阳性为7份。流感样病例中流行型别以A型为主,占59.6%;A型中甲型H1N1流感病毒核酸阳性为500例,占72.9%（500/686）,其中学生占84.8%（424/500）。10月-11月份达到流行高峰。随机抽取30份核酸阳性标本进行核酸序列测定,27份为甲型H1N1流感病毒。结论 2009年海南省流感样病例中甲型H1N1流感病毒感染主要以学生为主,开展流感病毒核酸检测,有助于尽早了解流感样病例中流感病毒感染情况,为及时了解甲型H1N1流感疫情的动态变化以及制定科学有效的甲型H1N1流感防控策略提供可靠的实验室依据。
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| 关 键 词： | 甲型H1N1流感病毒 核酸检测 流感样病例 |
Analysis of H1N1 influenza virus nucleic acid testing in Hainan Province in 2009 |
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| ZENG Xiang-jie,PAN Jia-xing,MA Yan,et al..Analysis of H1N1 influenza virus nucleic acid testing in Hainan Province in 2009[J].China Tropical Medicine,2011,11(10):1253-1254. |
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| Authors: | ZENG Xiang-jie PAN Jia-xing MA Yan |
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| Institution: | ZENG Xiang-jie,PAN Jia-xing,MA Yan,et al.(Hainan Provincial Center for Disease Control and Prevention,Haikou,570203,Hainan,China) |
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| Abstract: | Objective To know in time H1N1 influenza virus infection situation in flu-like illness and their close contacts of Hainan Province in 2009.Method Real-time fluorescent quantitative(RT-PCR) method was applied to qualitatively detect the influenza virus nucleic acid of nasopharyngeal swab specimens of respiratory tract.Result Annual sentinel surveillance and outbreak specimens were 1 148,the fluorescent quantitative PCR results were below:686 specimens were tested with nucleic acid type A influenza virus posi... |
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| Keywords: | H1N1 influenza virus Nucleic acid detection Influenza-like illness case |
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