| siRNA干扰Ezrin基因对人脐静脉内皮细胞增殖的影响 |
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| 引用本文： | 莫薇,袁竹青,王颖智,刘鷖雯,何怡青,高锋. siRNA干扰Ezrin基因对人脐静脉内皮细胞增殖的影响[J]. 热带医学杂志, 2011, 11(8): 870-874 |
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| 作者姓名： | 莫薇 袁竹青 王颖智 刘鷖雯 何怡青 高锋 |
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| 作者单位： | 1. 广州医学院病原生物学与免疫学教研室,广东广州,510182
2. 上海交通大学附属第六人民医院中心实验室,上海,200233 |
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| 基金项目： | 国家自然科学基金(81072491); 2009年度广州医学院博士、留学回国人员基金(L95116) |
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| 摘 要： | 目的探讨细胞膜与细胞骨架连接蛋白埃兹(Ezrin)对人脐静脉内皮细胞生长和转移能力的影响。方法培养人脐静脉内皮细胞,选择3对小干扰RNA(siRNA)序列下调Ezrin的表达,实时定量PCR和Western-blot印迹法分别检测Ezrin mRNA和蛋白表达水平的变化。BrdU ELISA法检测Ezrin siRNA干扰后细胞增殖能力的改变,伤口愈合实验检测Ezrin siRNA干扰后细胞迁移能力的变化,流式细胞术检测Ezrin siRNA干扰后细胞凋亡能力。结果实时定量PCR和Western-blot印迹结果显示,siRNA干扰后Ezrin的基因和蛋白水平均显著下调;BrdU法显示Ezrin siRNA干扰后细胞生长速率降低(P<0.05),划痕实验显示Ezrin siRNA干扰后细胞迁移率降低(P<0.05),流式细胞仪检测显示Ezrin siRNA干扰后细胞凋亡率升高。结论 Ezrin蛋白可影响脐静脉内皮细胞的增殖和迁移能力,推测其在血管功能和肿瘤研究中可能具有重要意义。
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| 关 键 词： | RNA干扰 Ezrin 人脐静脉内皮细胞 |
The effect of the knock-down of the Ezrin gene by siRNA on proliferation of HUVECs |
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| MO Wei,YUAN Zhu-qing,WANG Ying-zhi,LIU Yi-wen,HE Yi-qing,GAO Feng. The effect of the knock-down of the Ezrin gene by siRNA on proliferation of HUVECs[J]. Journal Of Tropical Medicine, 2011, 11(8): 870-874 |
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| Authors: | MO Wei YUAN Zhu-qing WANG Ying-zhi LIU Yi-wen HE Yi-qing GAO Feng |
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| Affiliation: | MO Wei1,YUAN Zhu-qing1,WANG Ying-zhi2,LIU Yi-wen2,HE Yi-qing2,GAO Feng2 (1.Department of Pathogen Biology and Immunology,Guangzhou Medical College,Guangdong,Guangzhou 510182,2.Department of Clinical Research Center,Shanghai Sixth People's Hospital,Shanghai Jiaotong University,Shanghai 200233,China) |
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| Abstract: | Objective To study the role of membrane-cytoskeleton linker Ezrin in the growth and migration of human umbilical vein endothelial cell (HUVEC).Methods HUVECs were cultured.Three pairs of Ezrin small interfering RNA(siRNA) were transfected into the HUVEC.RT-PCR and Western blotting were used to detect the expression of Ezrin mRNA and protein.The BrdU ELISA method was used for observing the effect of down-regulated Ezrin on HUVEC proliferation.Wound healing assay were used to detect the effect of down-regulat... |
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| Keywords: | RNA interference Ezrin human vascular endothelial cell in umbilical vein |
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