XS-1000i血液分析仪计数嗜碱性粒细胞影响原因分析

目的 分析Sysmex XS- 1000i血液分析仪计数嗜碱性粒细胞部分标本出现假性增高的影响原因.方法 对50例外周血中性粒细胞形态正常的标本使用Sysmex XE-5000、XS-1000i血液分析仪进行嗜碱性粒细胞计数,作相关统计学分析.4例使用Sysmex XS- 1000i血液分析仪计数嗜碱性粒细胞出现明显增高标本与手工计数法进行比较,做相关形态学分析.结果 50例外周血中性粒细胞形态正常的标本使用Sysmex XE-5000、XS-1000i血液分析仪进行嗜碱性粒细胞计数,两者相关系数r=0.715,嗜碱性粒细胞计数差异具统计学意义(P＜0.01).4例使用Sysmex XS-1000i血液分析仪计数嗜碱性粒细胞出现明显增高标本均出现中性粒细胞中毒颗粒、退行性变等形态学改变.结论 两仪器间计数嗜碱性粒细胞结果差异有统计学意义,外周血中性粒细胞中毒颗粒等形态改变会造成XS-1000i血液分析仪计数嗜碱性粒细胞假性增高,对嗜碱性粒细胞异常增高的结果需进行手工计数分类.

An investigation on the accuracy of Sysmex XS-1000i automated hematology analyzer in counting basophils

Objective To identify the cause underlying the false increase in basophil count tested by Sysmex XS-1000i automated hematology analyzer. Methods 50 peripheral blood samples with normal neutrophil morphology were compared between Sysmex XE-5000 and XS-1000i analyzers for the count of basophils. 4 blood samples with obvious increase in the basophil count tested by Sysmex XS-1000i automated hematology analyzer, were compared with the results from manual analysis. Results The correlation test about basophil rat...