Schistosoma japonicum,S. mansoni,S. haematobium,S. intercalatum,and S. rodhaini: cysteine-class cathepsin activities in the vomitus of adult worms |
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Authors: | Conor R. Caffrey Christian E. Rheinberg Hélène Moné Joseph Jourdane Yong-Long Li A. Ruppel |
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Affiliation: | Institut für Tropenhygiene, Universit?t Heidelberg, Im Neuenheimer Feld 324, D-69120 Heidelberg, Germany Fax: (+49) 6221-565948 Tel.: (+49)-6221–565044, DE Centre de Biologie et d’écologie Tropicale et Méditerranéenne, Université, Avenue de Villeneuve, F-66860 Perpignan Cedex, France, FR Department of Parasitology, Tongji Medical University, Hangkong Lu 13, 430030 Wuhan, P.R. China, CN
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Abstract: | Vomitus from adults of five Schistosoma species was screened for biochemical homologues of the mammalian cysteine proteinases cathepsins B, H, and L. Bovine cathepsin B and rat cathepsin L served as references. Using the substrate Arg-NMec, a schistosome cathepsin H-like activity was never detected. All species degraded the cathepsin B substrate Z-Arg-Arg-NMec, but distinct species differences were observed with respect to pH optima and buffer preferences. The cathepsin B and L substrate Z-Phe-Arg-NMec was similarly degraded by all species, and activity was abolished by the cysteine proteinase inhibitor E-64. Preferences by vomitus proteinase activities for Z-Phe-Arg-NMec over Z-Arg-Arg-NMec were similar to or higher than those found for bovine cathepsin B but well below those observed for rat cathepsin L; also, the preferential cathepsin L inhibitor Z-Phe-PheCHN2 only partially inhibited proteinolytic activity. The results suggest the possible presence in vomitus of a minor cathepsin L-like activity and demonstrate a major cathepsin B-like activity that is biochemically variable between schistosome species. Received: 1 April 1996 / Accepted: 19 June 1996 |
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