| 中波紫外线对HaCaT细胞凋亡、细胞周期变化及p16、c-myc蛋白表达的影响及阿魏酸干预作用研究 |
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| 引用本文： | 林秉奖,闵玮,骆丹.中波紫外线对HaCaT细胞凋亡、细胞周期变化及p16、c-myc蛋白表达的影响及阿魏酸干预作用研究[J].中国中西医结合皮肤性病学杂志,2010,9(1):8-11. |
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| 作者姓名： | 林秉奖 闵玮 骆丹 |
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| 作者单位： | 南京医科大学第一附属医院,南京,210029 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 目的观察传统中药川芎的有效成份阿魏酸(ferulic)对中波紫外线(UVB)辐射引起的人表皮角质形成细胞系HaCaT细胞凋亡、细胞周期及对p16、c-myc蛋白水平变化的影响,以探讨其光保护作用与机制。方法培养HaCaT细胞,以不同剂量UVB和200mg/mL浓度的阿魏酸处理细胞,以流式细胞仪检测0、30、60和90mJ/cm~2的UVB照射后24h细胞周期和凋亡率变化;以Western blot方法检测30mJ/cm~2UVB照射后p16、c-myc蛋白的表达水平。结果UVB照射诱导HaCaT细胞产生凋亡,凋亡率呈UVB剂量增加而升高;30mJ/cm~2剂量下细胞可出现明显S期阻滞,但高剂量时S期细胞数量相对下降;照光后p16、c-myc蛋白水平均增高。加入阿魏酸处理可抑制UVB引起的上述改变。结论阿魏酸可明显抑制UVB引起的凋亡与细胞周期阻滞以及p16、c-myc蛋白表达。
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| 关 键 词： | 中波紫外线 角质形成细胞 阿魏酸 凋亡 细胞周期阻滞 p16 c-myc |
Effects of Ferulic on Ultraviolet-B Irradiation Induced Apoptosis,Cell Cycle Arrest and Protein Expression of p16 and c-myc in Human Keratinocytes |
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| LIN Bing-jiang,MIN Wei,LUO Dan First Affiliated Hospital of Nanjing Medical University,Nanjing ,China.Effects of Ferulic on Ultraviolet-B Irradiation Induced Apoptosis,Cell Cycle Arrest and Protein Expression of p16 and c-myc in Human Keratinocytes[J].Chinese J of Dermatovenerology Integr Tradit and West Med,2010,9(1):8-11. |
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| Authors: | LIN Bing-jiang MIN Wei LUO Dan First Affiliated Hospital of Nanjing Medical University Nanjing China |
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| Institution: | LIN Bing-jiang,MIN Wei,LUO Dan First Affiliated Hospital of Nanjing Medical University,Nanjing 210029,China |
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| Abstract: | Objective To evaluate the effects of ultraviolet-B(UVB)irradiation and intervention of ferulic on apoptosis, cell cycle,and also expression levels of p16 and c-myc protein in human eternal keratinocyte-HaCaT cells.Methods Subconfluent HaCaT cells were shammed or irradiated with different dosages of UVB irradiation and treated with ferulic.The cell cycle and apoptotic rate were determined with a DNA flow cytometer at 24h after 0,30,60 and 90mJ/cm2 UVB irradiation.Western blot assay was used to measure protei... |
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| Keywords: | p16 c-myc |
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