复方丹参对兔肝脏热缺血再灌注损伤保护作用的实验研究

目的 探讨复方丹参对兔肝脏热缺血再灌注损伤的保护作用及其相关机制.方法 采用Pringle's肝脏缺血再灌注模型,48只SD大鼠随机分成三组:假手术组(A组),肝脏缺血冉灌注组(B组)和复方丹参预处理组(c组).测定各组缺血30 min(T1)、再灌注120 min(T2)肝功能(ALT、AST)、肝组织的抗氧化能力(SOD)、脂质过氧化产物丙二醛(MAD)含量、肝指数(湿重/干重%),比较肝组织形态学改变.结果 (1)T1、T2相,B、C组与A组比较肝组织损害重:ALT、AST均升高(P<0.05);肝组织匀浆SOD含量降低;MDA含量升高(P<0.05);肝脏水肿程度上升(P<0.05);肝组织出现不同程度的病理学改变.2)在T1、T2相,C组与B组比较肝组织损害轻:ALT、AST均较低(P<0.05);肝组织匀浆SOD含量高,MDA含量减少(P<0.05);肝脏水肿程度较轻(P<0.05);肝组织出现较轻的病理学改变.结论 复方丹参通过改善肝脏微循环状况,改善缺血肝脏组织的能量代谢,提高肝组织抗氧化能力,减少肝脏细胞的变性坏死程度而对肝脏热缺血再灌注起保护作用.

Experimental Studies on the Protective Effect of Salvia Miltiorrhia Bunge Against Hepatic Ischemia-reperfusion Injury

Objective To investigate the protective effect and mechanism of salvia miltiorrhiza bunge against ischemia-reperfusion injury on rabbits liver. Methods Forty-eight rabbits were randomly divided into three groups (16 rabbits in each group); group A: normal control;group B:ischemic-reperfusion model; group C:pretreated the ischemic-reperfusion model with salvia miltiorrhia bunge. Results (1)Compared to group A,in groups B and C,the injury of liver was found by pathological examination; MDA,ALT,AST and water content of liver tissues increased. SOD decreased in the two groups(P<0.05). (2)The serum ALT and AST levels in group C were much lower than those in group B(P<0.05); The pathological changes in group B was more serious than in group C(P<0.05). Conclusion Salvia miltiorrhia bunge pretreatment can prevent and reduce the hepatic injury from ischemic reperfusion,its mechanism is associated with restraining platelet aggregation, avoiding thrombus forming, increasing blood stream, ameliorating microcirculation, and improving the energy metabolism of the ischemic liver tissue.