Detection of non-HLA-DR determinants by alloreactive lymphocyte clones |
| |
Authors: | A Zeevi K Annen C Scheffel R J Duquesnoy |
| |
Affiliation: | Histocompatibility Research Laboratory. The Blood Center of Southeastern Wisconsin, Inc., Milwaukee, USA |
| |
Abstract: | Using the soft-agar colony assay, we have generated three MT3-associated clones: HJ1, HJ13, and HJ39, from an MLR combination of two unrelated individuals. Another clone, HJ37, appeared to recognize a novel HLA-D determinant. PLT inhibition studies with monoclonal anti-Ia-like antibodies (Mab) were conducted on clones HJ1, HJ39, and HJ37. Five different anti-DR Mab had no significant inhibitory effect on these clones. On the other hand, two Mab SG171 and Q5/13 which appear to react with DR and MT3 (I-A like) molecules strongly inhibited the two MT3-specific PLT clones. While SG171 and Q5/13 had little effect on HJ37, it was observed that a polymorphic Mab 17.15 had a strong inhibitory effect. These results, in concordance with biochemical data on Ia molecules precipitated by these Mab, suggest that these alloreactive clones may recognize non-DR PLT determinants. They also provide further indirect support that MT3 molecules represent the human homologue of murine I-A molecules. |
| |
Keywords: | Mab monoclonal antibody PLT primed lymphocyte testing MLR mixed leukocyte culture reaction TCGF T cell growth factor TCM tissue culture medium |
本文献已被 ScienceDirect 等数据库收录! |
|