MMP-2和PTEN蛋白在胃癌组织中的表达及临床意义

目的]探讨胃癌组织中MMP-2（基质金属蛋白酶-2）和PTEN（张力蛋白和辅助蛋白同源、第10号染色体丢失的磷酸酶基因）蛋白的表达与其恶性生物学行为的关系。方法]用免疫组化SP法检测64例胃癌组织和20例癌旁组织中MMP-2和PTEN蛋白的表达，并对随访的33例胃癌患者进行生存期分析。结果]MMP-2阳性表达率在胃癌和癌旁组分别为69．0％和40．5％（P〈0．05）；在高中分化和低分化胃癌组分别为54．5％和48．4％（P〉0．05），在侵及浆膜层和未侵及浆膜层组分别为61．5％和36．0％（P〈0．05），淋巴结转移和未转移组分别为68．3％和21．7％（P〈0．05），在生存期〈5年和生存期≥5年组分别为75．0％和50．0％（P〈0．05）；PTEN蛋白阳性表达率在胃癌和癌旁组分别为77．5％和91．2％（P〈0．05）。在高中分化和低分化胃癌组分别为51．5％和45．2％（P〉0．05），在侵及浆膜层和未侵及浆膜层组分别为52．5％和41．7％（P〉0．05），在淋巴结转移和未转移组的表达分别为26．8％和78．3％（P〈0．01），在生存期〈5年和生存期≥5年组分别为25．0％和58．8％（P〈0．05）。胃癌组织的MMP-2与PTEN蛋白表达呈显著负相关。结论]胃癌的MMP-2和PTEN蛋白的表达能反应其恶性潜能，并可作为判断预后的辅助指标。

Clinical study of relationship between expression of MMP-2 and PTEN in gastric cancer

Objective] To explore the relationship of the expressions of MMP-2 and PTEN of its maliguant behaveirs in gastric cancer. Methods] The expressions of MMP-2 and PTEN in 64 gastric carcinoma tissues were detected by immunohistochemistry SP method, and 33 patients were follavied were up. Results] The positive rates of MMP-2 in carcinoma and its surrounding tissue groups were 69.0% and 40.5% respectively (P<0.01); 54.5% and 48.2% in well and poor differentiated groups respectively (P>0.05); 61.5% and 36.0% in the groups with and without seraum infiltration respectively (P<0.05); 68.3% and 21.7% in gastric tissues with lymph node and without lymph node metastasis; 75.0% and 58.8% in patient whose survival periods were less than 5 yeas and survival periods were more than 5 yeas. The positive rates of PTEN in carcinoma and its surrounding tissue groups were 77.5% and 91.2% respectively (P<0.05); 51.5% and 45.2% in well and poor differentiated groups respectively (P>0.05); 52.5% and 41.7% in the groups with and without seraum infiltration respectively (P>0.05); 26.8% and 78.3% in gastric tissues with lymph node and without lymph node metastasis; 58.8% and 25.0% in patient whose survival periods were less than 5yeas and survival periods were more than 5 yeas. The expression of MMP-2 correlated negatively with that of PTEN. Conclusion] The expression of MMP-2 and PTEN was closely associated with lymph node metastasis and prognosis; detecting the expression of MMP-2 and PTEN may be helpful for predicting prognosis in gastric carcinoma.