| 大黄酸对大鼠前体脂肪细胞增殖与分化的影响 |
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| 引用本文： | 杨永青,杨公社.大黄酸对大鼠前体脂肪细胞增殖与分化的影响[J].中国药学杂志,2007,42(15):1148-1151. |
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| 作者姓名： | 杨永青 杨公社 |
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| 作者单位： | 1. 西北农林科技大学动物脂肪沉积与肌肉发育实验室,陕西,杨凌,712100;山西师范大学生命科学学院,山西,临汾,041000
2. 西北农林科技大学动物脂肪沉积与肌肉发育实验室,陕西,杨凌,712100 |
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| 基金项目： | 国家重点基础研究发展计划(973计划);国家自然科学基金 |
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| 摘 要： | 目的研究大黄酸(rhein,Rh)对大鼠前体脂肪细胞增殖与分化的影响。方法采用噻唑蓝(MTT)比色法和流式细胞术测定RH对大鼠前体脂肪细胞增殖的影响。采用油红O染色提取法,检测脂肪细胞内甘油三酯积聚量的变化;采用形态学观察,检测前体脂肪细胞充脂率、充脂程度及形态变化。结果RH在5～160μmol·L-1内,对前体脂肪细胞增殖与分化的抑制作用呈剂量和时间依赖性变化,并可在一定程度上诱发脂肪细胞凋亡。结论Rh可抑制前体脂肪细胞的增殖与分化,具有潜在的减肥降脂作用。
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| 关 键 词： | 大黄酸 前体脂肪细胞 增殖与分化 凋亡 |
| 文章编号： | 1001-2494（2007）15-1148-04 |
| 收稿时间： | 2006-04-18; |
| 修稿时间： | 2006-04-18 |
Effect of Rhein on Proliferation and Differentiation of Rat Preadipocytes |
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| YANG Yong-qing,YANG Gong-she.Effect of Rhein on Proliferation and Differentiation of Rat Preadipocytes[J].Chinese Pharmaceutical Journal,2007,42(15):1148-1151. |
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| Authors: | YANG Yong-qing YANG Gong-she |
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| Institution: | 1. Laboratory of Fat Deposition and Muscle Development, North West Sci-tech University of Agriculture and Foristry , Yangling 712100, China; 2. College of Life Science, Shanxi Normal University, Linfen 041000, China |
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| Abstract: | OBJECTIVE To study the effect of Rh(rhein) on proliferation and differentiation of rat preadipocytes.METHODS MTT spectrophotometry and FCM(flow cytometry) were adopted to determine the effect of Rh on proliferation of rat preadipocytes. The accumulation of TG(triglyceride) in adipocytes was assayed by oil red O staining,and morphological changes of the adipocytes were determined by morphology observation. RESULTS Rh inhibited the proliferation and differentiation of preadipocytes in a dose and time dependent manner,and induced apoptosis of preadipocytes in a certain degree. CONCLUSION Rh has a potential to block the excessive development of adipose tissue in human or animal’s body,so that it can be used to treat obesity and lipid metabolic disorder. |
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| Keywords: | rhein preadipocytes proliferation and differentiation apoptosis |
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