胰岛素样生长因子-1诱导神经干细胞向少突胶质细胞分化研究

目的探讨胰岛素样生长因子（IGF），1对多能神经干细胞分化的影响。方法取3～5代神经干细胞培养至对数生长期后接种于多聚赖氨酸包被的盖玻片上，在含有或无IGF-1的培养基条件下贴壁培养7～10d，进行免疫组织化学检测和免疫荧光显色，观察分化为少突胶质细胞、神经元及星形细胞的数量，进行对照分析。结果神经干细胞在不含IGF-1培养基的条件下分化7～10d，分化为少突胶质细胞的数量较少，而在含有IGF-1的培养基的条件下分化为少突胶质细胞的数量明显增加。结论IGF-1可以诱导神经干细胞向少突胶质细胞分化。

Differentiation of newborn rat hippocampus-derived neural stem cells into oligodendrocytes by insulin-like growth factor I

Objective To explore the effect of insulin-like growth factor I (IGF-1) on the differentiation of newborn rat hippocampus-derived neural stem cells (NSCs). Methods The NSCs were isolated from the hippocampus of newborn Wistar rats. The cells differentiated in the conditions without IGF-1 or with IGF-1 for 7-10 days. Cells were labeled with rabbit anti-RIP,rabbit anti-MBP,mouse anti-MAP2 and rabbit anti-GFAP, respectively. The number of oligodendroytes, neurons and astrocytes was calculated for comparison. Results If the NSCs differentiated in the conditions with IGF-1 for 7-10 days,they would differentiate into a large percentage of RIP + or MBP + cells. There was statistically significant difference between no-IGF-1 group and IGF-1 group. Conclusion IGF-I can induce the differentiation of NSCs into oligodendrocytes.