| 3-脱氧葡萄糖醛酮体外生成AGEs模型的建立 |
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| 引用本文： | 江国荣,张露蓉,唐文敏.3-脱氧葡萄糖醛酮体外生成AGEs模型的建立[J].抗感染药学,2008,5(3):158-160. |
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| 作者姓名： | 江国荣 张露蓉 唐文敏 |
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| 作者单位： | [1]南京中医药大学附属苏州中医医院、苏州市中医药研究所,江苏苏州215003 [2]南京中医药大学、2006级硕士研究生,江苏南京210029 |
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| 基金项目： | 苏州市高层次人才资助项目（编号：2004-33） |
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| 摘 要： | 目的：建立可用于药物筛选的体外蛋白质非酶糖基化模型。方法：37℃孵育1周后用流动注射法于波长（λ：Ex／Em）：370／440nm处测定糖基化终产物（AGEs）。结果：反应1周后，反应体系内3-脱氧葡萄糖醛酮（3．DG）浓度与AGEs的吸收度值呈线性关系，y=22．946x＋22．846；r^2=0．9985。结论：随着各反应体系中底物3-DG浓度的增加及反应时间的延长，蛋白质非酶糖基化反应增强3-DG为底物与牛血清白蛋白组成反应系统，能较可靠地反映AGEs的生成变化，有望成为体外蛋白质非酶糖基化的1种新模型。
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| 关 键 词： | 3-脱氧葡萄糖醛酮 糖基化终产物 流动注射分析 |
Establishment of the Reaction System for Advanced Glycation End Products Induced by 3-deoxyglucosone in vitro |
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| Institution: | JIANG Guo-rong , ZHANG Lu-rong, TANG Wen-ming(1.Suzhou Hospital of Traditional Chinese Medicine, Suzhou Jiangsu 215003, China 2.Nanjing University of Traditional Chinese Medicine, Nanjing Jiangsu 210029, China) |
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| Abstract: | Objective:To establish the reaction of nonenzymatic glycation of protein.Methods:BSA(bovine serum albumin)was incubated with 3-DG(3-deoxyglucosone)for one week at 37℃in vitro.AGEs(advanced glycation end products)was estimated by FIA atλ370/440 nm(excitation/emission).Results:AGEs were increased accompanying with the increasing of 3-DG,showing positive linear relations(v=22.946x+22.846;r~2=0.998 5).Conclusions:The present study showed that the reaction of nonenzymatic glycation of protein was reinforced with the concentration increasing and time lengthening of 3-deoxyglucosone.Furthermore,our results supported the notion that the reacting systems,composing 3-deoxyglucosone incubatirig with bovine serum albumin,might become a novel model for researching AGEs formed in vitro due to their characters of monitoring the variances of AGEs sensitively. |
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| Keywords: | 3-DG(3-deoxyglucosone 3-DG) AGEs(advanced glyeation end products AGEs) FIA(flow injection assay) |
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