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| 快速测定cyp2d6~*10等位基因类型的PCR法 | |
| 引用本文： | 马涛,蔡卫民,卜莹,陈冰,周国华. 快速测定cyp2d6~*10等位基因类型的PCR法[J]. 中国药理学通报, 2007, 23(7): 973-976 |
| 作者姓名： | 马涛 蔡卫民 卜莹 陈冰 周国华 |
| 作者单位： | 1. 徐州医学院麻醉药理学教研室,江苏,徐州,221002 2. 上海第二医科大学附属瑞金医院药剂科,上海,200025 3. 华东医学生物技术研究所,江苏,南京,210002 |
| 摘 要： | 目的建立一种简单易行经济的方法检测cyp2d6*10等位基因类型,研究其多态性在中国人群中的分布情况。方法建立新的PCR扩增法,并以经典的PCR-RFLP法与之对比,用所建立的新方法测定224份标本。结果两种方法的结果完全一致,标本cyp2d6*10等位基因多态性的分布频率与报道相似。结论所建立的新方法准确、简单,为临床个体化用药提供遗传学依据。 |
| 关 键 词： | 聚合酶链反应 单核苷酸多态性 cyp2d6*10 基因分布频率 |
| 文章编号： | 1001-1978(2007)07-0973-04 |
| 修稿时间： | 2007-01-28 |
A rapid PCR method to determine the types of cyp2d6*10 alleles |
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| MA Tao,CAI Wei-min,BU Ying,CHEN Bing,ZHOU Guo-hua. A rapid PCR method to determine the types of cyp2d6*10 alleles[J]. Chinese Pharmacological Bulletin, 2007, 23(7): 973-976 | |
| Authors: | MA Tao CAI Wei-min BU Ying CHEN Bing ZHOU Guo-hua |
| Abstract: | Aim To establish a new simple, easy and economical PCR method for determining cyp2d6~*10 allele and studying its distributive frequencies in Chinese population. Methods The new PCR are established and compared with the classical PCR-RFLP. The 224 samples have been determined with the new PCR.Results the results of the two methods go on the way. The allele distribution frequencies of cyp2d6~*10 resembles the reports. Conclusion the new method is proved to be accurate and convenient. It provides a genetics basis for the drug therapy with individuals in clinics. |
| Keywords: | cyp2d6*10 |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |