槲皮素对人鼻咽癌HEN1细胞系增殖抑制和诱导凋亡作用的研究

目的:目的:探讨黄酮类化合物槲皮素对人鼻咽癌HEN1细胞系的抑制增殖和诱导凋亡的作用,并对其机制做初步探讨。方法:应用MTT法检测不同浓度槲皮素对人鼻咽癌HEN1细胞的增殖抑制作用;应用流式细胞仪检测细胞凋亡率及细胞周期分布;比色法测定凋亡相关蛋白caspase-3的表达。结果:槲皮素能明显抑制人鼻咽癌HEN1细胞增殖,存在剂量依赖(r=0.709;P<0.01)与时间依赖(r=0.703;P<0.01);HEN1细胞经不同浓度槲皮素(15、30、60、90、120μmol/L)作用48 h后细胞凋亡率分别为4.81%、9.02%、14.33%、19.65%、28.88%,明显高于对照组(1.70%)(P<0.01);随着槲皮素浓度升高,凋亡细胞和坏死细胞比例增加,将细胞特异性地阻滞在G2/M期,出现凋亡峰;槲皮素组的HEN1细胞凋亡相关因子caspase-3表达明显高于对照组(P<0.05)。结论:槲皮素能通过caspase-3途径抑制人鼻咽癌HEN1细胞增殖,诱导细胞凋亡,并具有细胞周期特异性。

Effect of quercetin on proliferation and apoptosis of human nasopharyngeal carcinoma HEN1 cells

Objective:To investigate the effect of quercetin(Que) on HEN1 cells,a kind of human nasopharyngeal carcinoma cells.Method:Inhibition rate of quercetin on HEN1 was assayed by MTT method,cell apoptosis by flow cytometry(FCM),Caspase-3 expression of each group were determined by colorimetry set.Result:The inhibition of quercetin on HEN1 cells was shown in the dose-dependent(r=0.709,P<0.05) and time-dependent manner(r=0.703,P<0.01).The ratio of apoptotic and necrosis cells increased accompanied with added quercetin concentration.Cell cycle was specificly arrested inG2/M phase.Apoptosis cusp was revealed by FCM.While the activity of caspase-3 were also significantly upregulated in five groups after quecetin compared to control(P<0.05).Conclusion:Quecetin can activate the expression of Caspase-3 and induce the apoptosis of HEN1 cells through mitochondrion-depended pathway.