| 复频聚焦超声激活血卟啉杀伤离体肿瘤细胞的实验研究 |
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| 引用本文： | 尚志远,张军平,朱旭宁,齐浩,刘全宏,张坤.复频聚焦超声激活血卟啉杀伤离体肿瘤细胞的实验研究[J].中国生物医学工程学报,2004,23(5):433-437. |
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| 作者姓名： | 尚志远 张军平 朱旭宁 齐浩 刘全宏 张坤 |
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| 作者单位： | 1. 陕西师范大学物理学与信息技术学院、应用声学研究所,西安,710062
2. 陕西师范大学,生命科学学院,西安,710062 |
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| 基金项目： | 国家自然科学基金项目资助 ( 19874 0 4 2 ) |
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| 摘 要： | 在优选的超声辐照参数下 ,利用复频聚焦超声激活血卟啉 (Hp)杀伤肿瘤细胞K5 6 2 ,SW - 4 80并用MTT法检测分析了杀伤细胞的效率其初步结果为 :单频超声杀伤结果时低频优于高频 ;复频和单频比较 ,复频优于单频。而且杀伤效率不是单频的代数和 ,而是二者和的 2 - 3倍 ;由超声辐照后的孵育时间可以看出 :孵育 16h超声 Hp的杀伤效率高于 4h。
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| 关 键 词： | 复频聚焦超声 血卟啉 MTT法 细胞凋亡 |
| 文章编号： | 0258-8021(2004)-05-433-05 |
| 修稿时间： | 2003年7月15日 |
EXPERIMENTAL STUDY ON KILLING TUMOUR CELLS BY BI-FREQUENCY FOCAL ULTRASOUND ACTIVATED HEMATOPORPHYRIN IN VITRO |
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| SHANG Zhi-yuan , ZHANG Jun-ping , ZHU Xu-ning ,QI Hao , LIU Quan-hong ZHANG Kun.EXPERIMENTAL STUDY ON KILLING TUMOUR CELLS BY BI-FREQUENCY FOCAL ULTRASOUND ACTIVATED HEMATOPORPHYRIN IN VITRO[J].Chinese Journal of Biomedical Engineering,2004,23(5):433-437. |
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| Authors: | SHANG Zhi-yuan ZHANG Jun-ping ZHU Xu-ning QI Hao LIU Quan-hong ZHANG Kun |
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| Institution: | SHANG Zhi-yuan 1, ZHANG Jun-ping 1, ZHU Xu-ning 1,QI Hao 2, LIU Quan-hong 2 ZHANG Kun 2 |
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| Abstract: | Tumor cells K562 and SW-480 were killed by bi-frequency focal ultrasound activated hematoporphyrin(Hp)under the optimum irradiation parameter of ultrasound.The cell-killing effect was investigated by MTT method.Result indicated that lower frequency ultrasound is more effective than higher in killing tumor cells.The bi-frequency ultrasound exhibited an improved effect of killing tumor cells,than single,which is 2-3 times of the effect induced by single frequency ultrasound.Under the irradiation of ultrasound,the cell-killing effect of hatching 16h is better than that of hatching 4h. |
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| Keywords: | Bi-frequency focal ultrasound Hematoporphyrin MTT method Cell qpopsis |
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