| 小鼠谷胺酸脱羧酶65基因克隆的初步报告 |
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| 引用本文： | 邬艳慧,李强,王丽宏,孙玉倩,梁玮,张巾超.小鼠谷胺酸脱羧酶65基因克隆的初步报告[J].中华糖尿病杂志,2006,14(6):461-463. |
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| 作者姓名： | 邬艳慧 李强 王丽宏 孙玉倩 梁玮 张巾超 |
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| 作者单位： | 哈尔滨医科大学附属第二医院内分泌科,150086 |
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| 基金项目： | 黑龙江省哈尔滨市科技攻关项目 |
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| 摘 要： | 目的克隆小鼠谷胺酸脱羧酶05（GAD65）基困。方法（1）刚RT-PCR的方法扩增小鼠GAD65基因。（2）将GAD65克隆到T载体PUCm-T上。（3）对质粒PUCm-T／GAD65进行酶切鉴定和序列分析。结果克隆的DNA片段为1758bp的小鼠GAD65的全基因编码序列。结论成功的克隆了小鼠GAD65摹因，为构建GAD65基因疫苗以及精尿病的免疫干预治疗打下基础 。
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| 关 键 词： | 谷胺酸脱羧酶 克隆 |
| 收稿时间： | 2005-09-16 |
| 修稿时间： | 2005-09-16 |
Preliminary report of cloning of murine glutamic acid decarboxylase 65 |
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| WU Yan-hui, LI Qiang, WANG Li hong , et al.Preliminary report of cloning of murine glutamic acid decarboxylase 65[J].CHINESE JOURNAL OF DIABETES MELLITUS,2006,14(6):461-463. |
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| Authors: | WU Yan-hui LI Qiang WANG Li hong |
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| Institution: | Department of Endocrinology, The Second Affiliated Ho.spital of Harbin Medical University, Harbin 150086, China |
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| Abstract: | Objective To construct a clone of murine GAD65. Methods(1)Mouse cDNA coding GAD65 was synthesized and amplified using RT-PCR system.(2)The cDNA was then connected with the T vector PUCm-T.(3)The PUCm-T/GAD65 clone was examined by incision enzyme and sequence analysis.Results A clone of complete murine GAD65 gene was constructed. Conclusions A clone of murine GAD65 is successfully constructed.It is a basis step for further construction of expression plasmid that expresses GAD65 and for immunologic intervention of T1DM. |
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| Keywords: | Glutamic acid decarboxylase Cloning |
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