检测病毒性肝炎患者血清中SEN病毒及其临床意义

目的：检测病毒性肝炎患者血清中SEN病毒D和H(SENV-D、SENV-H),并探讨其临床意义。方法：采用巢式聚合酶链反应法（nPCR）检测甲、乙、丙、戊型肝炎和非甲－戊型肝炎患者血清中SENV-D和SENV-H DNA。结果：在180例病毒性肝炎患者血清中，SENV-D和SENV-H检出率分别为17.2%(31/180)和5.6%(10/180)，总检出率为18.3%(33/180)、甲、乙、丙、戊型肝炎患者的SENV-D/H检出率高于非甲－戊型肝炎患者。从甲、乙、丙、戊型肝炎和非甲－戊型肝炎患者分离的SENV-D/H核苷酸序列，与SENV-D/H原型株比较，其同源性在94％以上。甲、乙、丙和戊型肝炎患者有无SENV-D/H合并感染，其血清生化学指标无明显差异。结论：SENV-D/H可能不是非甲-戊型肝炎的病原，甲、乙、丙和戊型肝炎患者合并感染SENV-D/H并不加重病情。

SEN virus and its clinical significance in patients with viral hepatitis

Objective To detect SEN virus D and H (SENV D, SENV H) in serum from patients with viral hepatitis and its clinical significance. Methods SENV D and SENV H were detected by a nest polymerase chain reaction(nPCR) in the sera of patients with hepatitis A, B, C, E and non A to E (HAV, HBV, HCV, HEV and non A E). Results Of 180 patients with viral hepatitis including 30 acute hepatitis A, 30 chronic hepatitis B, 30 chronic hepatitis C, 30 acute hepatitis E, and 60 non A to E hepatitis, 31(17.2%) were SENV D positive, and 10(5.6%)were SENV H positive. The total positive rate of SENV D/H was 18.3% (33/180). The SENV D/H positive rate of patients with HAV, HBV, HCV and HEV hepatitis was higher than that with non A E hepatitis. The nucleotide homology between SENV H/D prototype and isolates from patients with viral hepatitis was more than 94%. No difference was found in biochemical markers between hepatitis A, B, C, E patients with SENV D/H infection and the patients without SENV D/H infection. Conclusion SENV D/H is not a pathogenic agent of non A E hepatitis, and SENV D/H coinfected with HAV, HBV, HCV and HEV does not contribute to the severity of the diseases. [