Determination of antibodies and non-specific immunoglobulins produced by single cells

To determine whether one cell simultaneously produces antibodies and non-specific immunoglobulins (nIg), spleen cells from mice immunized twice with SRBC were cultivated on membrane filters, firstly on the layer of native SRBC in agarose and then on the layer of SRBC sensitized with anti-mouse IgG antibodies. A specific immunoadsorbent, eliminating all anti-SRBC antibodies, was placed between the filters with spleen cells and sensitized SRBC. By comparing the location of indirect and reverse plaques (i.e. the location of cells producing antibodies to SRBC and nIg) it was shown that antibody-forming cells do not synthesize nIg.