辅助性T17细胞在牙周炎小鼠中的免疫状态研究

目的 研究辅助性T（Th）17细胞在牙周炎小鼠中的免疫状态。方法 将7周龄C57BL/6雌性小鼠随机分为牙周炎组和对照组，每组4只。牙周炎组采用口腔涂抹牙龈卟啉单胞菌（P. gingivalis）的方法建立牙周炎动物模型。对照组涂抹PBS液。在涂抹结束后的第4周取材，流式细胞仪检测CD4+维甲酸相关核孤儿受体（ROR）γτ+（Th17）细胞；酶联免疫吸附（ELISA）检测Th17细胞相关的细胞因子白细胞介素（IL）-17A的蛋白表达。结果 牙周炎小鼠牙龈组织、颈部淋巴结和外周血中CD4+ RORγτ+（Th17）细胞在总CD4+ T细胞中的比例和细胞数量显著高于对照组（P<0.01）。与对照组相比，牙周炎组IL-17A的表达增加（P<0.05）。结论 在牙周炎的发生发展中，Th17细胞介导的细胞免疫应答增强，牙龈组织、颈部淋巴结和外周血可能是Th17细胞介导免疫应答的主要场所。

Study on the immune status of T helper 17 cells in mice with periodontitis

Objective This study investigates the immune status of T helper (Th) 17 cells in mice with periodontitis. Methods Seven-week-old C57BL/6 female mice were randomly divided into the control and periodontitis groups. Each group comprisedfour mice. Experimental periodontitis was induced in mice by oral infection with Porphyromonas gingivalis in the periodontitis group. Phosphate-buffered saline solution was used in the control group. All mice were sacrificed 4 weeks after the last P. gingivalis infection. CD4+ retinoid-related orphan receptor (ROR) γτ+ (Th17) cells were analyzed by flow cytometry. The protein expression of Th17 cell-related cytokine interleukin (IL)-17A was detected by enzyme-linked immunosorbentassay (ELISA). Results Flow cytometry showed that the percentage of CD4+ RORγτ+ (Th17) cells in all CD4+T cells and the cell number in gingival tissues, cervical lymph nodes (CLNs), and the peripheral blood obviously increased (P<0.01) in the periodontitis group. ELISA showed that compared with the control group, the periodontitis group exhibited an obvious increase in the protein expression of IL-17A (P<0.05). Conclusion Th17 cell-mediated cell response is enhanced, and the gingival tissues, CLNs, and the peripheral blood are probably the main locations of Th17 cell-mediated cell response during the development of periodontitis.