左心辅助泵(人工心)内皮细胞化的实验研究

培养猪主动脉内皮细胞,用预衬细胞外基质结合使用bFGF的方法实现静置条件下PU材料的内皮细胞化,并观察内皮化材料在LVAP泵腔内模拟流体条件下内皮细胞的贴壁率和丢失率以及内皮细胞的形态变化。结果显示用预衬基质结合使用bFGF的方法,静置条件下可以实现PU材料的内皮化。镜下观察细胞形态良好,在LVAP泵腔内模拟流体条件下冲击1、2h后,细胞贴壁率分别为74.42%和59.7%,丢失率分别为25.58%和40.30%。扫描电镜观察,细胞总的形态变化不明显,轻度收缩,部分细胞有脱落。本实验为实现LVAP泵腔内壁流体条件下内皮化初步摸索了条件和实验方法。

A Study on the Endothelialization Mechanism of Left Ventricular Pump

The cultural model of porcine aortic endothelial cell(EC) was set up. By combined use of basic fibroblast growth factor(bFGF) with extracellular matrix(ECM), the endothelialization on PU under static culture was accomplished and the attachment rate as well as the morphological changes of EC under flowing shear stress condition were observed when LVAP was working at normal blood circulation pressure(12 KPa).The result revealed that EC treated with bFGF grew very well on the PU material precoated with fibronectin and collagen IV. Under shear stress condition for 1 and 2 hours, the percentage of cell attachment attained 74.42% and 59.7%. The general morphological changes of the cell were not obvious under the scanning electron microscope, slight shrink and cell death or loss were found. This work was done to find out the condition and method of experiment for the accomplishment of endothelialization under the flowing shear stress in the cavity of LVAP.