化疗对急性白血病外周血NO、SOD、OFR及MDA影响的探讨

目的 :探讨化疗对急性白血病 (AL)外周血一氧化氮 (NO)、超氧化物歧化酶 (SOD)、氧自由基 (OFR)及丙二醛 (MDA)的影响。方法 :对 4 6例 AL患者的外周血 ,采用比色法检测其 NO、SOD、OFR和 MDA的水平变化 ,并与正常人进行比较。结果 :AL患者外周血 NO水平与正常对照差异无显著性 (P>0 .0 5 ) ,而 SOD、OFR水平明显低于正常对照组 (P<0 .0 1) ,MDA水平化疗前明显高于正常对照组 (P<0 .0 1) ,化疗药物对 AL 外周血中各个时期的影响差异无显著性 (P>0 .0 5 )。结论 :NO在急性白血病发病过程中的影响还需进一步商榷 ,SOD和 OFR低水平状态及 MDA水平升高提示参与了急性白血病的发病过程 ,化疗药物对患者外周血中的 NO、SOD、OFR和 MDA水平的变化影响不大。

Study the effect of chemotherapy on nitric oxide,superoxide dismutase,oxygen free radical and malonic aldehyde in peripheral blood of patients with acute leukemia

Objective:To study the effect of chemotherapy on nitric oxide(NO),superoxide dismutase(SOD),oxygen free radical(OFR) and malonic aldehyde(MDA) in peripheral blood of patients with acute leukemia(AL).Methods:Colorymetries were used to determine NO,SOD,OFR and MDA levels in peripheral blood of 46 patients and 30 normal controls.Results:The level of NO was no significant difference in peripheral blood between the patients with acute leukemia and controls(P>0.05).The levels of SOD and OFR in peripheral blood of the patients were significantly lower than that of controls(P<0.01),and the level of MDA in peripheral blood of patients before treatments was significantly higher than that of controls(P<0.01).The chemotherapy drugs had no significantly difference on the levels of NO,SOD,OFR and MDA in peripheral blood of patients with AL in all periods(P>0.05).Conclusion:The effect of NO on the process of patients fall ill with acute leukemia is yet to be discussed.The changes of SOD,OFR and MDA indicated that they participated in the occurrence of AL.The chemotherapy drugs had no significantly different effect on the levels of NO,SOD,OFR and MDA in peripheral blood of patients with acute leukemia.