白芷、川芎药对配伍挥发油成分的GC-MS分析

目的 研究白芷、川芎单味药及其药对的挥发油成分，并考察白芷、川芎药对配伍对挥发油成分的影响。方法 采用水蒸气蒸馏法提取白芷、川芎单味药及其药对的挥发油，运用气相色谱-质谱联用技术，结合计算机检索，并与文献资料比对，进行化学成分的分离和鉴定。色谱条件：TR5-MS毛细管色谱柱（30 m× 0.25 mm，0.25 μm）；载气为高纯氦气；进样口温度250℃；进样量1 μL，分流比50：1；恒流模式，载气流速1.00 mL/min；程序升温：起始温度50℃，保持1 min，以5℃/min 的速率升至240℃，保持1 min，然后以5℃/min 升至280℃，并保持1 min。质谱条件：电子轰击（EI）离子源，电子能量70 eV，离子源温度220℃，传输线温度280℃，质量范围（m/z）：50～650 amu，数据采集扫描模式为全扫描，溶剂延迟5 min。结果 在白芷、川芎、药对（1：1）、药对（4：1）的挥发油及白芷、川芎等浓度挥发油混合物（药对等浓度挥发油）中分别鉴定出69、59 、59、60、78 种化合物，其中共有成分19种。结论 从挥发油的组分及含量来看，不同配伍比例药对挥发油的成分有很大的不同，单味药挥发油组分的含量在药对中都发生了变化。

GC-MS in analysis of chemical constituents of volatile oils in drug pair of Radix Angelica dahurica and Ligusticum chuanxiong Hort.

Objective To use ultrasound radio-frequency (RF) technology for investigating atherosclerosis degree difference between the left and right common carotid arteries (CCA) in patients with type 2 diabetes, and to analyze the related factors. Methods The compliance coefficient (CC) and pulse wave velocity (PWV) of the left and right common carotid arteries (CCA) were obtained by ultrasound RF technology. The ratios of the left to right CCAs were calculated: CCratio and PWVratio. The difference within groups was observed by left and right comparison and that between groups was observed by comparison of the same side. The relevant factors of CCratio and PWVratio were analyzed. Results (1) PWV of the left CCA was significantly higher than that of the right one and CC was significantly lower than that of the right one in patients with type 2 diabetes (P<0.05). The CC and PWV were not significantly different between the left and right CCAs in the control group. (2) Compared with the control group, patients with type 2 diabetes had significantly increased left PWV and right CC (P<0.05). (3) PWVratio and CCratio were significantly different between the two groups (P<0.05). Multiple linear regression analysis showed that age, disease course, triglycerides and systolic blood pressure (SBP) were the independent risk factors for PWVratio; and age, HDL-C were the independent risk factor for CCratio. Conclusion The atherosclerosis degrees are different between the left and right CCA in patients with type 2 diabetes. The age, disease course, SBP, triglyceride and HDL-C are independent risk factors for the differences.