| CpG ODN联合光动力疗法对肝癌移植瘤生长及局部CD8+细胞毒性T细胞的影响 |
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| 引用本文： | 张南征,赵霞. CpG ODN联合光动力疗法对肝癌移植瘤生长及局部CD8+细胞毒性T细胞的影响[J]. 中国激光医学杂志, 2008, 17(6) |
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| 作者姓名： | 张南征 赵霞 |
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| 作者单位： | 解放军第九七医院消化内科,徐州市,221004 |
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| 基金项目： | 南京军区"十一五"医药卫生科研基金重点课题资助项目 |
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| 摘 要： | 目的 探讨以CpG寡脱氧核苷酸(ODN)为免疫佐剂联合光动力疗法(PDT)的光免疫疗法(PIT)对小鼠Heps肝癌移植瘤的抗肿瘤及免疫效应.方法 92只ICR小鼠皮下接种肝癌Heps细胞建立荷瘤鼠模型,随机分为对照组、PDT组、CpG ODN组和PIT组,治疗后定期测量各组肿瘤体积,计算抑瘤率.免疫组化法计数肿瘤局部CD8+细胞毒性T细胞(CD8+CTLs).结果 (1)与埘照组相比,PDT组、CpG ODN组和PlT组治疗后16d肿瘤体积均明显缩小(P<0.01),抑瘤率分别为73.88%、20.09%、84.47%.PIT组肿痛体积较单纯PDT组明显缩小(P<0.05).(2)免疫组化染色结果显示,PDT组、CpG ODN组和PIT组治疗后2、24h肿瘤局部CD8+CTLs数量较对照组无明显变化(P>0.05),治疗后72h肿瘤局部CD8+CTLs数量,均明显高于对照组(P<0.05);PIT组肿瘤局部CD8+CTLs数量明显高于单纯PDT组(P<0.01).结论 CpG ODN能激活宿主免疫应答而有效增强PDT对小鼠Heps肝癌移植瘤的抗肿瘤及免疫效应.
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| 关 键 词： | 光动力疗法 CpG寡脱氧核苷酸 CD8 细胞毒性T细胞 Heps肝癌 小鼠 |
Effect of Photodynamic Therapy Combined with CpG ODN on Tumor Growth and CD8+CTL of Transplanted Hepatoma |
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| ZHANG Nan-zheng,ZHAO Xia. Effect of Photodynamic Therapy Combined with CpG ODN on Tumor Growth and CD8+CTL of Transplanted Hepatoma[J]. Chinese Journal of Laser Medicine & Surgery, 2008, 17(6) |
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| Authors: | ZHANG Nan-zheng ZHAO Xia |
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| Affiliation: | ZHANG Nan-zheng,ZHAO Xia Department of Gastroenterology,the 97th Hospital of PLA,Xuzhou,Jiangsu 221004,China |
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| Abstract: | Objective To investigate the anti-tumor and immune efficacy of photodynamic therapy(PDT) combined with CpG oligodeoxynucleotide(CpG ODN) on murine Heps hepatoma.Methods Hepatoma model was established by subcutaneous inoculation with Heps cells into the right dorsal area in ICR mice,then they divided into four groups at random: control group,PDT group,CpG ODN group and photoimmunotherapy(PIT) group.Tumor sizes were measured regularly after treatment.The number of CD8+cytotoxic T lymphocytes(CTL) infiltrated ... |
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| Keywords: | Photodynamic therapy CpG ODN CD8+CTLs Heps hepatoma Mice |
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