心肺复苏后大鼠神经细胞凋亡的实验研究

目的：研究全脑缺血再灌注后大鼠脑神经细胞的凋亡情况。方法：Sprague Dawley 大鼠28只，雌雄各半，随机分5组，即对照组(假手术组)以及复苏后3、6、12、24h组，对照组4只，其余每组6只。采用窒息合并冰氯化钾停跳液致大鼠心跳骤停-心肺复苏模型，停跳5min后开始心肺复苏，采用TUNEL方法和透射电镜观察复苏后神经细胞的凋亡情况。结果:复苏后3h大鼠脑皮质神经元就出现细胞凋亡，复苏后24h海马区TUNEL阳性率为6．0％。结论:细胞凋亡可能是大鼠全脑缺血再灌注后神经细胞损伤的主要机制之一。

Experimental study on apoptosis of neuronal cell after cardiopulmonary resuscitation in rats

Objective To investigate the apoptosis of neuronal cell after global ischemia-reperfusion.Methods Cardiac arrest was induced by asphyxiation and ice-cold 0.5 M KCl in rats and resuscitation efforts would begin five munites after arrest. 28 Sprague Dawley rats, half female and half male, were randomly divided into 5 groups: sham control group (n=4); after cardiopulmonary resuscitation rats were allowed to reperfuse spontaneously for 3, 6, 12 and 24 hours(n=6,each group). The temporospatial cellular reactions were assessed by terminal deoxynucleotidyltransferase-mediated dutp-FITC nick endlabeling (TUNEL) and transmission electron microscope (TEM). Results TEM revealed early onset apoptosis in cortex 3 hours after resuscitation. At 12 hours post-resuscitation, TUNEL-positive cells could be observed in the hippocampal. There were 6.0% TUNEL-positive cells at 24 hours. Conclusion Apoptosis was one mechanism of neuronal cell damage after global ischemia-reperfusion.