耐甲氧西林金黄色葡萄球菌SCCmec基因分型研究与耐药基因检测

目的了解耐甲氧西林金黄色葡萄球菌(MRSA)的葡萄球菌盒染色体(SCCmec)基因型分布特点和耐药基因的携带状况。方法采用多重聚合酶链反应(PCR)对医院分离的125株MRSA进行SCCmec基因型分型,普通PCR对其中的50株MRSA进行6种耐药基因扩增。结果检测的125株MRSA中124株SCCmec基因型为Ⅲ型,1株不能分型,6种耐药基因的阳性率分别为aac(6′)/aph(2″)98%、aph(3′)Ⅲ基因46%t、etM基因72%、erm基因86%,未扩增出TEM和ant(4′、4″)基因。结论引起感染的MRSA的基因型基本为SCCmecⅢ型,SC-CmecⅢ型的MRSA对氨基糖苷类、红霉素类、四环素类耐药基因有较高的携带率,多重PCR法可以有效的用于大批量临床分离的MRSA的SCCmec分型研究。

Genotyping of SCCmec and Detection of Antibiotic-resistant Genes in Meticillin-resistant Staphylococcus aureus

OBJECTIVE To investigate the staphylococcal cassette chromosome mec (SCCmec) genotype characteristics and antibiotic-resistant genes in meticillin-resistant Staphylococcus aureus (MRSA) isolated from Lianyungang. METHODS The SCCmec of clinically isolated MRSA strains were genotyped with a novel multiplex PCR strategy reported by Zhangetal. Antibiotic-resistant genes of aac(6')/aph(2"), aph(3') Ⅲ, tetM,erm,TEM,and ant(4',4") were analyzed by traditional PCR. RESULTS The isolates were almost SCCmec Ⅲ positve, only one isolate couldn't be typed. The positive rates of aac(6')/aph(2"),aph(3')Ⅲ ,terM, and erm were 98% ,46%,72% and 86%, respectively. TEM and ant(4',4") tested were all negative. CONCLUSIONS Almost all genotypes of MRSA prevailing in Lianyungang carry the SCCmec Ⅲ gene. There are high positive percentages of antibioticresistant genes of aac(6')/aph(2"), aph(3') Ⅲ, tetM and erm in the isolates. The novel multiplex PCR strategy recommended by Zhang et al can be applied into genotyping study of MRSA SCCmec effectively.