慢性应激抑郁模型大鼠海马cAMP反应元件结合蛋白的表达及氟西汀的干预作用

目的 研究慢性应激抑郁模型大鼠海马cAMP反应元件结合蛋白(CREB)的表达情况及氟西汀的干预作用.方法 将大鼠随机分为抑郁模型组、氟西汀治疗组和对照组.模型组和氟西汀组给予21d的应激刺激,此期间对照组正常饲养,刺激期间氟西汀组每天腹腔注射氟西汀(10mg/kg),模型组和对照组每天腹腔注射等体积的生理盐水.行为学检测应用open-field法和液体消耗实验.采用免疫组织化学法和Western-blotting法检测各组大鼠海马CREB的表达情况.结果 应激后,模型组水平穿越格数[(8.2 ±2.7)格、竖立次数(8.1±3.5)次、修饰次数(4.3±1.6)次]、糖水消耗百分比[(52.5 ±7.8)%]均显著低于对照组[分别为(31.3±5.8)%、(13.9 ±3.2)%、(10.6 ±2.4)%、(68.3 ±4.5)%;均P＜0.01].应激后氟西汀组水平穿越格数[(15.3±7.7)格]低于对照组(P＜0.01),但高于模型组(P＜0.05);竖立次数[(8.2±5.6)次]低于对照组(P＜0.01),与模型组差异无显著性(P＞0.05);修饰次数[(6.2 ±1.5)次]低于对照组(P＜0.01),但高于模型组(P＜0.05);糖水消耗百分比(66.7 ±5.1)%与对照组差异无显著性(P＞0.05),但高于模型组(P＜0.05).免疫组织化学检测和Western-blotting法检测中,模型组大鼠海马CREB的表达均显著低于对照组(P＜0.01);氟西汀组大鼠海马CREB的表达均显著低于对照组(P＜0.01),但高于模型组大鼠(P＜0.01).结论 慢性应激抑郁模型大鼠海马中CREB的表达降低并可以被氟西汀部分逆转.

The expression of CREB in hippocampus of chronic stress depression rat and the interference of fluoxetine

Objective To research the expression of CREB in hippocampus of chronic stress depression rat and the interference of fluoxetine to the CREB. Methods All the experimental rats were divided by random into 3 groups:depression group, fluoxetine group and control group. The rats of depression group and fluoxetine group were applied stress for 21 days, and meanwhile the rats of control group were fed normally. The rats of fluoxetine group were applied fluoxetine by peritoneal injection(10mg/kg), and the rats of another groups were given normal sodium of the same volume by peritoneal injection. The ethology examination was performed by using method of open-field and experiment of fluid consumption. The expression of CREB was detected by immunohistochemical method and Western-blotting. Results After the chronic stress, the horizontal crossing numbers, the erection times, the modification times and the percentage of sacchar-consumption of the rats of depression group were 8. 2 ±2. 7;8. 1 4-3. 5;4. 3 4-1. 6 and 52. 5%4-7. 8%respectively, which were less than control group(31. 3 4-5. 8;13. 9 ±3. 2;10. 6 ±2. 4 and 68. 3%±4. 5%;P＜0. 01). The horizontal crossing numbers(15. 3 ±7. 7)and themodification times(6. 2±1. 5)of fluoxetine group were less than those of control group(P＜0. 01), but more than depression group(P＜0. 05);the erection times(8. 2 ±5. 6), fluoxetine group was less than control group(P＜0. 01), but no obvious difference with depression group(P＞0. 05);the percentage of sacchar-consumption (66. 7%±5. 1%), fluoxetine group was more than depression group(P＜0. 05), but no significant difference compared with control group(P＞0. 05). In both immunohistochemical method and western-blotting, the level of CREB in the hippocampus of depression group was less than that of Control group(P＜0. 01), and the level of CREB in the hippocampus of fluoxetine group was less than that of Control group(P＜0. 01)but more than that of depression group(P＜0. 01). Conclusion The expression of CREB in hippocampus of chronic stress depression rat decreased and can be reversed by fluoxetine to some extent.