Determination of Matrix Metalloproteinases in Human Radicular Dentin |
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| Authors: | Juliana Santos Marcela Carrilho Taina Tervahartiala Timo Sorsa Lorenzo Breschi Annalisa Mazzoni David Pashley Franklin Tay Caio Ferraz Leo Tjäderhane |
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| Affiliation: | 1. Department of Restorative Dentistry, Endodontics Area, Piracicaba Dental School, University of Campinas, Piracicaba, SP, Brazil;2. Department of Restorative Dentistry, Dental Materials Area, Piracicaba Dental School, State University of Campinas, Piracicaba, SP, Brazil;3. GEO/UNIBAN, Bandeirante, University of São Paulo, School of Dentistry, São Paulo, SP, Brazil;4. Institute of Dentistry, University of Helsinki, Helsinki, Finland;6. Department of Oral and Maxillofacial Diseases, Helsinki University Central Hospital (HUCH), Helsinki, Finland;5. Department of Biomedicine, University of Trieste, Trieste, Italy;7. Department of Oral Biology, School of Dentistry, Medical College of Georgia, Augusta, GA;11. Institute of Dentistry, University of Oulu and Oulu University Hospital, Oulu, Finland |
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| Abstract: | Matrix metalloproteinases (MMPs) are present in sound coronal dentin and may play a role in collagen network degradation in bonded restorations. We investigated whether these enzymes can also be detected in root dentin. Crown and root sections of human teeth were powderized, and dentin proteins were extracted by using guanidine-HCl and EDTA. Extracts were analyzed by zymography and Western blotting for matrix metalloproteinases detection. Zymography revealed gelatinolytic activities in both crown and root dentin samples, corresponding to MMP-2 and MMP-9. MMP-2 was more evident in demineralized root dentin matrix, whereas MMP-9 was mostly extracted from the mineralized compartment of dentin and presented overall lower levels. Western blot analysis detected MMP-8 equally distributed in crown and root dentin. Because MMPs are also present in radicular dentin, their contribution to the degradation of resin-dentin bonds should be addressed in the development of restorative strategies for the root substrate. |
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