桂郁金提取物对人肝星状细胞增殖、细胞周期及凋亡影响的研究

目的观察桂郁金提取物对人肝星状细胞的作用,了解其对该细胞的增殖、细胞周期和凋亡情况的影响。方法将人肝星状细胞株(HSC-LX2)与药物孵育48 h后,用MTT比色法测定细胞的增殖抑制率。用流式细胞术(PI染色法)检测各周期细胞的DNA含量及细胞凋亡的概况。结果低、中、高浓度桂郁金提取物均可抑制HSC-LX2细胞的增殖,48h的增值抑制率分别为15.4%、49.1%、58.3%;48 h的IC50为45μmol/L;均可阻止HSC-LX2细胞进入DNA合成增殖周期,并诱导HSC-LX2细胞发生早期及晚期凋亡,48 h的S期+G2/M期细胞总数百分比分别为70.1%、63.6%、50.2%;凋亡概率分别为13.9%、18.9%、23.5%。结论桂郁金提取物具有抑制HSC-LX2细胞增殖、阻止细胞进入DNA合成增殖周期、诱导细胞发生凋亡的能力,从而可以干预肝纤维化病程;以上能力均具有剂量依赖性;且中、高剂量桂郁金提取物组以上能力明显比秋水仙碱组和复方鳖甲软肝片组强。

Effect of Guangxi Curcuma Extract on the Proliferation, Cell Cycle and Apoptosis in Human Hepatic Stellate Cell

Objective To observe the effects of Guangxi curcuma extract on human hepatic stellate cells.Methods The human hepatic stellate cells(HSC-LX2) were with the drugs for 48 h.Then the cell proliferation inhibition rate was measured by MTT,the DNA content and apoptosis were detected by flow cytometry(PI staining method).Results Low,medium and high concentrations of Guangxi curcuma extract could inhibit proliferation of HSC-LX2 cells.The inhibition rate in 48h was 15.4%,49.1% and 58.3% respectively.IC50 in 48 h was 45 μmol / L.All concentrations could prevent HSC-LX2 cells into the cycle of DNA synthesis and proliferation and induce early and late apoptosis of HSC-LX2 cells.The percentages of total cells in S phase + G2 / M cells in 48 h were 70.1%,63.6% and 50.2%,respectively.The apoptosis rates were 13.9%,18.9% and 23.5%,respectively.Conclusion Guangxi curcuma extract can inhibit the proliferation of HSC-LX2 cells,prevent cell cycle into DNA synthesis and proliferation,induce apoptosis,so as to interfere with course of the liver fibrosis.