参麦注射液联合川芎嗪对脓毒症小鼠肝脏的保护作用

目的探讨参麦联合川芎嗪注射液对脓毒症小鼠肝脏的保护作用。方法采用盲肠结扎穿孔(Cecal ligation and puncture,CLP)方法诱导形成脓毒症。将雄性成年的昆明小鼠随机分为5组,分别是假手术组、模型组、参麦注射液治疗组、川芎嗪注射液治疗组和参麦联合川芎嗪注射液治疗组。CLP术前24,12,1 h各组小鼠分别腹腔注射参麦注射液和川芎嗪注射液或者等计量的生理盐水。观察CLP造模后24 h各组小鼠血清中肿瘤坏死因子α(TNF-α)、白介素6(IL-6)含量、谷丙转氨酶(ALT)、谷草转氨酶(AST)活性以及肝脏组织中丙二醛(MDA)、髓过氧化物酶(MPO)水平,并观察各组小鼠的存活率及其肝脏组织的病理变化。结果参麦、川芎嗪及参麦联合川芎嗪三组药物治疗组的脓毒症小鼠其死亡率、血清中炎症因子(TNF-α、IL-6)含量、肝脂质过氧化(MDA、MPO)水平、ALT/AST及其肝脏病理损伤程度均较模型组明显减轻,尤以参麦联合川芎嗪给药治疗组疗效最为显著。此外,联合给药治疗组血清中的炎症因子(TNF-α、IL-6)水平较单一用药组显著降低。结论参麦联合川芎嗪注射液较单一用药能够更有效地抑制炎症反应,提高脓毒症小鼠的存活率、减轻肝脏损伤。

Protective Effect of Shenmai Combined with Ligustrazine Injection on Liver Iniury in Sepsis Mice

Objective To investigate the protective effect of Shenmai injection combined with Ligustrazine injection on sepsis-induced liver injury in mice.Methods Sepsis-induced liver injury was induced by cecal ligation and puncture(CLP) in KunMing mice.Male mice were randomly divided into sham operation group(group A),model group(group B),Shenmai injection group(group C),Ligustrazine injection group(group D) and Shenmai combined Ligustrazine injection group(group E).Animals received a separate intraperitoneal injection of Shenmai and Ligustrazine injection or normal saline at 24h,12h and 1h before CLP.Results The survival rate of mice was significantly increased and pathological injury was distinctively allectated in the group C,D and E as compared to group B.And in these three treatment groups,the levels of ALT,AST,IL-6,TNF-α in serum and MPO,MDA in hepatic tissue were lowered obviously,as compared with group B.Shenmai combined with Ligustrazine injection showed the best therapeutic action to sepsis mice.Furthermore,in group E the levels of serum pro-inflammatory cytokine(TNF-α,IL-6)were remarkably decreased as compared with group C and D.Conclusion These findings demonstrated that Shenmai combined with Ligustrazine injection can effectively resist systemsatic inflammation,attenuate liver injury and improve the survival rate of sepsis mice.