精浆超氧化物歧化酶活性与精子活力的关联分析

[目的]探讨精浆SOD活性与精子活力的相关性以及SOD活性用于临床诊断的可行性.[方法]194例精子活力正常和228例精子活力低下的男性进行精液常规分析,通过Dojindo四氮唑盐(WST-1)底物比色法和二喹啉甲酸法(BCA)对其精浆SOD活性及精浆总蛋白含量进行测定.[结果]每毫升精浆中SOD活性在两组中没有明显差异,但是每毫克精浆蛋白中SOD活性在低精子活力组中显著下降(P＜0.0001).SOD活性在正常对照组和低精子活力组中均有较大的个体差异.ROC曲线分析表明两组之间没有显著的阈值区分(Az=0.644).[结论]精浆SOD活性与精子活力具有显著相关性,但是临床利用SOD活性进行弱精症诊断的准确性较低.

An association analysis of seminal SOD activity and sperm motility

Objective To investigate the association of seminal SOD activity with sperm motility and the possibility of clinical diagnosis using SOD activity assay.Methods Semen parameters of 194 subjects with normal sperm motility and 228 subjects with low sperm motility were analyzed in the reproduction medicine center of our hospital.Individual seminal SOD activity and seminal protein concentration were determined by colorimetric assay using Dojindo WST-1 substrate and by BCA method.Results There was a significant decrease in mean SOD activity measured in units per mg of seminal protein in subjects with low sperm motility compared to controls(P<0.0001),but no significant difference of SOD activity in units per ml of seminal plasma was found between controls and cases.Large variations in individual SOD activity existed in both controls and cases,and ROC curve analysis showed no threshold value could be identified to separate semen samples with normal and low sperm motility.Conclusions The seminal SOD activity is significantly associated with sperm motility.However,it is difficult to apply SOD activity for asthenozoospermia diagnosis in clinic due to its low predictability.