白术药材质量标准研究

目的建立白术中白术内酯类成分的含量测定方法。方法采用高效液相色谱(HPLC)法测定不同产地的生白术及蜜麸炒白术药材,Lichrospher C18(4.6 mm×150 mm id,5μm)色谱柱,乙腈-0.1%甲酸水(62∶38)。流速为1.0 ml/min,白术内酯I、III的检测波长为220 nm、白术内酯II检测波长为270 nm。结果得到了12批生白术、11批蜜麸炒白术中白术内酯Ⅰ,Ⅱ,Ⅲ的含量。结论按干燥品计算,生白术白术内酯Ⅰ,Ⅱ,Ⅲ不得低于0.25,0.31,0.38 mg.g-1;蜜麸炒白术白术内酯Ⅰ,Ⅱ,Ⅲ不得低于0.17,0.20,0.25 mg.g-1。

Quality Control of Atractylodes macrocephala Koidz

Objective To determine atractylenolides Ⅰ、Ⅱ、Ⅲ in Rhizoma Atractylodes Macrocephalae by HPLC.Methods RP-HPLC method was used with Lichrospher C18(4.6 mm×150 mm id,5μm)column and the mobile phase was composed of acetonitrile-water(62:38)with a isocratic elution.The flow rate was 1.0ml·min-1 with DAD detected at 270 nm for atractylenolides Ⅱ and 220 nm for atractylenolide Ⅰ and Ⅲ.The column temperature was maintained at 25℃.Results The contents of atractylenolides Ⅰ,Ⅱ,Ⅲ were received in 12 batches on Rhizoma Atractylodes mac rocephalae and 11 batches parching on Rhizoma Atractylodes macrocephalae.Conclusion Rhizoma Atractylodes Macrocephalae contains not less than 0.25mg of atractylenolides Ⅰ per gram,0.31 mg of atractylenolides Ⅱ per gram,0.38 mg of atractylenolides Ⅲ per gram;parching Rhizoma Atractylodes macrocephalae contains not less than 0.17mg of atractylenolides I per gram,0.20 mg of atractylenolides Ⅱ per gram,0.25 mg of atractylenolides Ⅲ per gram,with reference to the dried drug.