4-羟基苯并恶唑-2-酮对小鼠急性肝损伤的保护作用

目的研究4-羟基苯并恶唑-2-酮(4-hydroxy-2-benzoxazolone,简称HBOA)对四氯化碳所致小鼠急性肝损伤的保护作用,并探讨其疗效机制。方法采用腹腔注射四氯化碳(Carbon tetrachloride,CCl4)制备小鼠急性肝损伤模型,HBOA灌胃给药,检测小鼠血清中的丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)活性以及肝组织中超氧化物歧化酶(SOD)、丙二醛(MDA)和谷胱甘肽(GSH)含量,观察光镜下肝组织的病理变化。结果 HBOA能明显降低CCl4致急性肝损伤小鼠血清ALT、AST活性,同时升高肝组织中SOD、GSH的活性,降低肝组织MDA的含量;减轻肝组织坏死程度。结论 HBOA对CCl4所致小鼠急性肝损伤有一定的保护作用,其保护机制可能与其抗脂质过氧化有关。

Protective Effect of 4-Hydroxy-2-benzoxazolone against Acute Liver Injury in Mice

Objective To study the protective effect of 4-Hydroxy-2-benzoxazolone(HBOA) against liver injury induced by carbon tetrachloride(CCl4) in mice. Methods The acute liver injury model was prepared by the intraperitoneal injection of carbon tetrachloride.The levels of ALT,AST in serum and levels of MDA,SOD and GSH in liver tissue were measured and hepatic histological changes were observed by optical microscope in order to evaluate the protective effects of HBOA. Results Compared with control group,the levels of serum ALT,AST and MDA in liver tissue were decreased significantly,the levels of SOD,GSH in liver tissue were increased obviously.Histological results showed that HBOA could ameliorate histological damages induced by CCl4 in liver. Conclusion HBOA has protective effects against acute hepatic injury induced by CCl4 in mice.