Effects of copper on A-type potassium currents in acutely dissociated rat hippocampal CA1 neurons |
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Authors: | Niu Zhi-Dian Yu Kuai Gu Yan Wang Ming She Jia-Qi Chen Wei-Heng Ruan Di-Yun |
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Affiliation: | Department of Neurobiology and Biophysics, School of Life Science, University of Science and Technology of China, Anhui, PR China. |
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Abstract: | The effects of copper on voltage-gated A-type potassium currents were investigated in acutely dissociated rat hippocampal CA1 neurons using the whole-cell patch-clamp technique. Extracellular application of various concentrations of copper (1-1000 microM) reversibly reduced the amplitude of voltage-gated A-type potassium currents in a dose-dependent manner with a 50% inhibitory concentration value of 130 microM. Copper (300 microM) increased the V1/2 of the activation curve and state-inactivation curve by 17.2 and 9.0 mV, respectively. Thus, copper slowed down the activation and inactivation process of voltage-gated A-type potassium currents. This study indicated that copper reversibly inhibits the hippocampal CA1 neuronal voltage-gated A-type potassium current in a dose-dependent and voltage-dependent manner, and such actions are likely involved in the regulation of the neuronal excitability and the pathophysiology of Wilson's disease. |
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