| 高效毛细管电泳法测定莱菔子中芥子碱含量 |
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| 引用本文： | 蔡梅超.高效毛细管电泳法测定莱菔子中芥子碱含量[J].辽宁中医学院学报,2011(9):45-46. |
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| 作者姓名： | 蔡梅超 |
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| 作者单位： | 山东中医药大学,山东济南250355 |
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| 基金项目： | 国家新药研发大平台项目（2009ZX09301-013）;山东省自然科学基金项目（ZR2010HM017）;山东省教育厅科技计划项目（J105LL05）;山东省科技发展计划项目（2008GG2NS02011） |
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| 摘 要： | 目的：测定中药莱菔子中芥子碱的含量。方法：采用高效毛细管电泳法,电泳条件为石英毛细管柱（75μm×90cm,实际长度81cm）,运行缓冲液为0.02mol/L Na2HPO4：0.02mol/L NaH2PO4=1：1（25℃时pH=6.89）,分离电压为25kV,压力进样30mbar×8s,温度为25℃,检测波长为326nm。结果：芥子碱在0.2～1.0mg/mL范围内呈良好的线性关系（r=0.9995）,平均回收率为99.50%,RSD为1.21%。结论：高效毛细管电泳法可用于测定莱菔子中芥子碱的含量。
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| 关 键 词： | 莱菔子 芥子碱 高效毛细管电泳法 |
Determination of Sinapine in Semen Raphani by High Performance Capillary Eiectrophorsis |
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| CAI Mei-chao.Determination of Sinapine in Semen Raphani by High Performance Capillary Eiectrophorsis[J].Journal of Liaoning College of Traditional Chinese Medicine,2011(9):45-46. |
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| Authors: | CAI Mei-chao |
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| Institution: | CAI Mei-chao ( Shandong University of Traditional Chinese Medicine, Ji＇nan 250355, Shandong, China ) |
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| Abstract: | Objective：To determine the content of Sinapine in Semen Raphani.Methods：Apply the methods of HPCE,and the best condition was using the quartz capillary tube（75μm×90cm,practical length is 81cm）,0.02mol/L Na2HPO4：0.02mol/L NaH2PO4=1：1（pH=6.89 at 25℃）buffer,pressure injection 30mbar× 8s,at a constant voltage of 25kV and temperature at 25℃.It was detected on 238nm.Results：Sinapine had a good linearity within 0.2～1.0mg·mL^-1（r=0.9995）,and the average recovery were 99.50% with RSD 1.21%.Conclusion：This method is feasible for determination of Sinapine in Semen Raphani. |
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| Keywords: | Semen Raphani Sinapine HPCE |
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