白英水提物对胃癌细胞凋亡与Notch1信号通路的影响

目的：观察白英水提物对胃癌SGC-7901细胞凋亡及Notch1基因表达的影响。方法：常规法制备白英水提物,实验分正常对照组、白英处理组。白英处理组加入白英水提物终浓度分别为12.5、25mg/mL和50mg/mL。MTT法检测细胞增殖抑制率,流式细胞仪检测细胞凋亡,半定量RT-PCR和免疫化学分析细胞中Notch1、caspase-9基因表达。结果：与正常对照组比较,白英处理组SGC-7901细胞增殖有显著性下降,细胞凋亡显著性增多（P〈0.05）,Notch1基因表达呈显著性的下降（P〈0.05）、caspase-9基因表达有明显的上调（P〈0.05）,并随白英浓度增加而加强。结论：白英水提物能下调人胃癌SGC-7901细胞中Notch1信号蛋白表达和促进细胞凋亡,这可能是其发挥抗癌作用的重要机制之一。

Effects of Solanum Lyratum Thumb Extract on Notchl Gene Expression and Apoptosis in Human Stomach SGC-7901 Cells

Objective：To investigate the effects of Solanum Lyratum Thunb Extract（SLT）on apoptosis and the expression of Notch1,Caspase-9 genes in Human stomach cancer SGC-7901 cells.Methods：Dried whole herbs of Solanum Lyratum Thunb were extracted by boiling distilled water.Human Stomach Cancer SGC-7901 Cells were randomly divided into the control group and SLT-treated groups（12.5mg/mL,25mg/mL,50mg/mL）,the growth inhibitory rate was evaluated by MTT assay,cell apoptosis rate was determined by flow cytometry;Expression of Notch1,Caspase-9 mRNA were detected by semi-quantitive RT-PCR,Expression level of Notch1 and Caspase-9 proteins were detected by two-step immunhistochemical staining.Results：Compared with control group,the proliferation inhibitory rate and apoptosis rate were increased obviously（P〈0.05）,the expression of Notch1 mRNA and protein were obvious decreased respectively（P〈0.05）,the expression of Caspase-9 mRNA and protein were obvious increased respectively（P〈0.05）,and display a effect in a dose-dependent manner in the SGC-7901 cells of the SLT-treated groups.Conclusion：SLT can downregulate expression level of Notch1 and induce apoptosis of the Human stomach cancer SGC-7901 cells.